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Team:Chiba/Calendar-Home/12 September 2008
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(New page: >Home | Notebook 11 September 2008 <|[[Team:Chiba/Calendar-Home/13 September 2008|> 13 September 2008...) |
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混ぜ表 | 混ぜ表 | ||
| - | <table width=" | + | <table width="257" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> |
| - | <td width="257"></td> | + | <td width="257">Sample No.</td> |
| - | <td> | + | <td>①</td><td>②</td><td> ③</td><td> ④</td><td> ⑤</td> |
<tr> | <tr> | ||
| - | <td> | + | <td>Cultures(μL)</td> |
| - | <td>0μl</td><td> | + | <td>0μl</td><td>1.9μl</td><td>19μl</td><td>190μl</td><td>-</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td> 20%L-Arabinose(μL)</td> |
| - | <td>1.9μl</td><td> | + | <td>0μl</td><td>1.9μl</td><td>19μl</td><td>190μl</td><td>-</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>L-Arabinase conc.</td> |
| - | <td> | + | <td>0%(N.C)</td><td>0.2%</td><td>2%</td><td>20%</td><td>P.C</td> |
| - | + | ||
| - | + | ||
| - | <td> | + | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
</tr> | </tr> | ||
</table> | </table> | ||
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L-アラビノースを入れてから15min,1h,2h,6h,12h,24h後に蛍光強度を計測した。 | L-アラビノースを入れてから15min,1h,2h,6h,12h,24h後に蛍光強度を計測した。 | ||
| - | |||
| + | Result: | ||
<table width="400" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="400" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| - | <td width=" | + | <td width="2300">Time after arabinose induction / Sample No.</td> |
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td> | <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>30min</td> |
<td>8.890</td><td>9.752</td><td>10.10</td><td>9.413</td><td>9.701</td> | <td>8.890</td><td>9.752</td><td>10.10</td><td>9.413</td><td>9.701</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>1h</td> |
<td>9.161</td><td>13.93</td><td>17.37</td><td>14.97</td><td>10.60</td> | <td>9.161</td><td>13.93</td><td>17.37</td><td>14.97</td><td>10.60</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>2h</td> |
<td>9.417</td><td>27.96</td><td>40.25</td><td>29.87</td><td>10.72</td> | <td>9.417</td><td>27.96</td><td>40.25</td><td>29.87</td><td>10.72</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>6h</td> |
<td>9.933</td><td>74.99</td><td>150.9</td><td>125.0</td><td>10.04</td> | <td>9.933</td><td>74.99</td><td>150.9</td><td>125.0</td><td>10.04</td> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>12h</td> |
<td>10.64</td><td>99.39</td><td>202.88</td><td>72.08</td><td>11.76</td> | <td>10.64</td><td>99.39</td><td>202.88</td><td>72.08</td><td>11.76</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>24h</td> |
<td>14.66</td><td>105.6</td><td>447.3</td><td>33.31</td><td>17.11</td> | <td>14.66</td><td>105.6</td><td>447.3</td><td>33.31</td><td>17.11</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
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===Team:Communication=== | ===Team:Communication=== | ||
Revision as of 02:54, 25 October 2008
11 September 2008 <|> 13 September 2008
Contents |
Laboratory work
Team:Input
- Pbad/araCのL-アラビノースによるスイッチングテスト
I1304(Ptet-RBS-GFP,XL10G)×1(①)(ネガティブコントロール用),R0051(PcI)×4(②～⑤)のコロニーをつついて2ml培養。
37℃,12h後液が濁っていたら、ODをはかり、48穴deep wellに①～⑤の菌液をそれぞれ190μl入れた。
L-アラビノースを以下のような濃度になるように入れた。
混ぜ表
| Sample No. | ① | ② | ③ | ④ | ⑤ |
| Cultures(μL) | 0μl | 1.9μl | 19μl | 190μl | - |
| 20%L-Arabinose(μL) | 0μl | 1.9μl | 19μl | 190μl | - |
| L-Arabinase conc. | 0%(N.C) | 0.2% | 2% | 20% | P.C |
L-アラビノースを入れてから15min,1h,2h,6h,12h,24h後に蛍光強度を計測した。
Result:
| Time after arabinose induction / Sample No. | ① | ② | ③ | ④ | ⑤ |
| 30min | 8.890 | 9.752 | 10.10 | 9.413 | 9.701 |
| 1h | 9.161 | 13.93 | 17.37 | 14.97 | 10.60 |
| 2h | 9.417 | 27.96 | 40.25 | 29.87 | 10.72 |
| 6h | 9.933 | 74.99 | 150.9 | 125.0 | 10.04 |
| 12h | 10.64 | 99.39 | 202.88 | 72.08 | 11.76 |
| 24h | 14.66 | 105.6 | 447.3 | 33.31 | 17.11 |
Team:Communication
no work
Team:Output
no work
