Team:Chiba/Calendar-Home/12 September 2008

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==Laboratory work==
==Laboratory work==
===Team:Input===
===Team:Input===
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*Pbad/araCのL-アラビノースによるスイッチングテスト
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*Pbad/araC:L-Arabinose induction test
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I1304(Ptet-RBS-GFP,XL10G)×1(①)(ネガティブコントロール用),R0051(PcI)×4(②～⑤)のコロニーをつついて2ml培養。
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*BBa_I1304(Ptet-RBS-GFP,XL10G)×1(①)(ネガティブコントロール用)
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*BBa_R0051(PcI)×4(②～⑤)のコロニーをつついて2ml培養。
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37℃,12h後液が濁っていたら、ODをはかり、48穴deep wellに①～⑤の菌液をそれぞれ190μl入れた。
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#incubated cultures at 37 degrees for 12hours.
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#Measured OD<sub>600</sub>.
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L-アラビノースを以下のような濃度になるように入れた。
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Reaction mixtures were mixed in 48 well plate(deep well).
混ぜ表
混ぜ表

Revision as of 03:06, 25 October 2008

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11 September 2008 <|> 13 September 2008

Contents

Laboratory work

Team:Input

  • Pbad/araC:L-Arabinose induction test
  • BBa_I1304(Ptet-RBS-GFP,XL10G)×1(①)(ネガティブコントロール用)
  • BBa_R0051(PcI)×4(②～⑤)のコロニーをつついて2ml培養。
  1. incubated cultures at 37 degrees for 12hours.
  2. Measured OD600.


Reaction mixtures were mixed in 48 well plate(deep well).

混ぜ表

Sample No. ①② ③ ④ ⑤
Cultures(μL) 0μl1.9μl19μl190μl-
20%L-Arabinose(μL) 0μl1.9μl19μl190μl-
L-Arabinase conc. 0%(N.C)0.2%2%20%P.C

L-アラビノースを入れてから15min,1h,2h,6h,12h,24h後に蛍光強度を計測した。


Result:

Time after arabinose induction / Sample No. ①②③④⑤
30min 8.8909.75210.109.4139.701
1h 9.16113.9317.3714.9710.60
2h 9.41727.9640.2529.8710.72
6h 9.93374.99150.9125.010.04
12h 10.6499.39202.8872.0811.76
24h 14.66105.6447.333.3117.11

Team:Communication

no work

Team:Output

no work

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