Team:Chiba/Calendar-Home/14 September 2008

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(Difference between revisions)

Revision as of 03:18, 25 October 2008

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13 September 2008 <|> 15 September 2008

Laboratory work

Team:Communication

(13/9)--->Mini prep

Transformation

Competent cells : XL10Gold

[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)

--->(15/9)

Team:Output

Time Responce Liquid
Digestion test

[http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
[http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
[http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
[http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
BBa_I716542
[http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
[http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
[http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
[http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
[http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](x2)
[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]

Sample No.	each
DNA(digested sample)(μL)	3
Buffer2(x10)(μL)	1
dH₂O(μL)	6
EcoRⅠ(μL)	0.075
TOTAL(μL)	10

--> left at37 degrees for 1 hour

Gel check

Sample No.	each
DNA(digestion sample)(μL)	10
Loading Dye(μL)	2
TOTAL(μL)	12

@@ Line 10: / Line 10: @@
 :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
-:Competent cells : XL10G
+:Competent cells : XL10Gold
-::*BBa_T9002[http://partsregistry.org/Part:BBa_T9002](2007)
+::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
-::*Plac+RhlI+LVA
+::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
-::*Plac+CinI+LVA
+::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
 :--->(15/9)
@@ Line 43: / Line 43: @@
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>each</td>
 </tr>
 <tr>
-<td>DNA(digested sample)</td>
+<td>DNA(digested sample)(μL)</td>
 <td>3</td>
 </tr>
 <tr>
-<td>Buffer2(x10)</td>
+<td>Buffer2(x10)(μL)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>6</td>
 </tr>
 <tr>
-<td>EcoRⅠ</td>
+<td>EcoRⅠ(μL)</td>
 <td>0.075</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>10</td>
 </tr>
 </table>
--->37℃ 1hour
+--> left at37 degrees for 1 hour
 [[Team:Chiba/protocol/gelcheck|Gel check]]
@@ Line 73: / Line 74: @@
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>each</td>
 </tr>
 <tr>
-<td>DNA(digestion sample)</td>
+<td>DNA(digestion sample)(μL)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(μL)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>12</td>
 </tr>
 </table>