Team:Chiba/Calendar-Home/16 September 2008

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Revision as of 03:23, 25 October 2008

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15 September 2008 <|> 17 September 2008

Laboratory work

Team:Communication

(15/9)->

time-delay test
- conditions

strain
Senders:JW1908
Receiver:JW1908
temparature
37℃ and 30℃

Team:Output

Digestion test

[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(x2)
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(x5)
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(x2)
[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](RBS+cinR)

Sample No.	each
DNA(μL)	3
EcoRⅠ(μL)	0.083
PstⅠ(μL)	0.083
BSA(x10)(μL)	1
Buffer3(x10)(μL)	1
dH₂O(μL)	5
TOTAL(μL)	10

-->37℃ 1hour

Gel check

Sample No.	each
DNA(μL)	10
Loading Dye(μL)	2
TOTAL(μL)	12

Transformation

[http://partsregistry.org/Part:BBa_J37019 BBa_J37019](LuxI+RBS+LuxR)->no colony
[http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
[http://partsregistry.org/Part:BBa_J37020 BBa_J37020](LuxI+LuxR+pLux+GFP)
[http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

PCR

Venus YFP(ptet+LuxR+YFP)

Sample No.	YFP
DNA(μL)	1
Venus Fwd(μL)	5
Rev(VR)(μL)	5
Thermo pol Buffer(μL)	10
dNTP mix(μL)	10
Vent(μL)	1
dH₂O(μL)	68
TOTAL(μL)	100

@@ Line 20: / Line 20: @@
 *[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
 *[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
-*[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)
+*[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](RBS+cinR)
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
@@ Line 28: / Line 28: @@
 </tr>
 <tr>
-<td>DNA</td>
+<td>DNA(μL)</td>
 <td>3</td>
 </tr>
 <tr>
-<td>EcoRⅠ</td>
+<td>EcoRⅠ(μL)</td>
 <td>0.083</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstⅠ(μL)</td>
 <td>0.083</td>
 </tr>
-<td>BSA(x10)</td>
+<tr>
+<td>BSA(x10)(μL)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>Buffer3(x10)</td>
+<td>Buffer3(x10)(μL)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>10</td>
 </tr>
@@ Line 66: / Line 67: @@
 </tr>
 <tr>
-<td>DNA</td>
+<td>DNA(μL)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(μL)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>12</td>
 </tr>
@@ Line 94: / Line 95: @@
 </tr>
 <tr>
-<td>DNA</td>
+<td>DNA(μL)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>Venus Fwd</td>
+<td>Venus Fwd(μL)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>Rev (VR)</td>
+<td>Rev(VR)(μL)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>Thermo pol Buffer</td>
+<td>Thermo pol Buffer(μL)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>dNTP</td>
+<td>dNTP mix(μL)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>Vent</td>
+<td>Vent(μL)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>68</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>100</td>
 </tr>
 </table>