Team:Chiba/Calendar-Home/17 September 2008

From 2008.igem.org

(Difference between revisions)
(New page: >Home | Notebook 16 September 2008 <|[[Team:Chiba/Calendar-Home/18 September 2008|> 18 September 2008...)
(→Team:Output)
Line 19: Line 19:
===Team:Output===
===Team:Output===
[[Team:Chiba/protocol/transformation|Transformation]]
[[Team:Chiba/protocol/transformation|Transformation]]
-
*BBa_I13500(RBS+GFP)
+
*[http://partsregistry.org/Part:BBa_I13500 BBa_I13500](RBS+GFP)
-
*BBa_J37019(Luxpr+LuxR)-->no colony
+
*[http://partsregistry.org/Part:BBa_J37019 BBa_J37019](Luxpr+LuxR)-->no colony
-
<br>MINI Prep
+
 
-
*BBa_J37033(RBS+LuxR)
+
[[Team:Chiba/protocol/DNA_Purification/sigma|miniprepped]]
-
*BBa_J37020(Luxpr+LuxR+LuxR+GFP)
+
 
-
*BBa_J37032(pLux+GFP)
+
*[http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
-
<br>Digestion
+
*[http://partsregistry.org/Part:BBa_J37020 BBa_J37020](Luxpr+LuxR+LuxR+GFP)
-
*BBa_I7100(ptetR+RBS)
+
*[http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)
-
*BBa_S03119(ptet+LuxR)
+
 
 +
[[Team:Chiba/protocol/digestion|Digestion]]
 +
 
 +
*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](ptetR+RBS)
 +
*[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
*Ligation product(ptet+LasR)
*Ligation product(ptet+LasR)
*Ligation product(ptet+RhlR)
*Ligation product(ptet+RhlR)
Line 34: Line 38:
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>each</td>
<td>each</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA template</td>
+
<td>DNA template(μL)</td>
<td>25</td>
<td>25</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRⅠ(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ</td>
+
<td>PstⅠ(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA(x10)</td>
+
<td>BSA(x10)(μL)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ Buffer</td>
+
<td>EcoRⅠ Buffer(μL)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
<td>40</td>
<td>40</td>
</tr>
</tr>
</table>
</table>
-
<br>-->37℃ 1hour
+
 
-
<br>->Next day
+
-->Left at37 degrees for 1 hour.
 +
 
 +
-->Next day

Revision as of 03:29, 25 October 2008

>Home | Notebook

16 September 2008 <|> 18 September 2008

Laboratory work

Team:Communication

  1. strain
    Senders:JW1908
    Receiver:JW1908
  2. temparature
    37℃ and 30℃
  1. strain
    Senders:XL10GOLD
    Receiver:JW1908
  2. temparature
    37℃ and 30℃


Team:Output

Transformation

  • [http://partsregistry.org/Part:BBa_I13500 BBa_I13500](RBS+GFP)
  • [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](Luxpr+LuxR)-->no colony

miniprepped

  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](Luxpr+LuxR+LuxR+GFP)
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

Digestion

  • [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](ptetR+RBS)
  • [http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
  • Ligation product(ptet+LasR)
  • Ligation product(ptet+RhlR)
  • Ligation product(ptet+cinR)
Sample No. each
DNA template(μL) 25
EcoRⅠ(μL) 1
PstⅠ(μL) 1
BSA(x10)(μL) 4
EcoRⅠ Buffer(μL) 4
dH2O(μL) 5
TOTAL(μL) 40

-->Left at37 degrees for 1 hour.

-->Next day

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