Team:Chiba/Calendar-Home/18 September 2008

From 2008.igem.org

(Difference between revisions)
(New page: >Home | Notebook 17 September 2008 <|[[Team:Chiba/Calendar-Home/19 September 2008|> 19 September 2008...)
(→Team:Output)
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===Team:Output===
===Team:Output===
-
<br>SAP 1μl
+
[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] 1μL
-
<br>->37℃　1hour
+
 
-
<br>->30min　65℃
+
->left for:
-
<br>Zymo ->25μl
+
*1 hour at 37 degrees
-
<br>Ligation
+
*30 minutes at 65 degrees.
-
<br>vector:BBa_I7100
+
 
-
<br>insert:BBa_S03119(ptet+LuxR),Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
+
[[Team:Chiba/protocol/transformation|Zymoclean]]
 +
 
 +
->eluted with 25μL of NFW.
 +
 
 +
[[Team:Chiba/protocol/ligation/ligation|Ligation]]
 +
*vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
 +
*insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
 +
*Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
 +
 
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>Vector&insert</td><td>Vector</td>
<td>Vector&insert</td><td>Vector</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(vector)</td>
+
<td>DNA(vector)(μL)</td>
<td>3.5</td><td>3.5</td>
<td>3.5</td><td>3.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(insert)</td>
+
<td>DNA(insert)(μL)</td>
<td>7.5</td><td>-</td>
<td>7.5</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase</td>
+
<td>Ligase(μL)</td>
<td>1</td><td>1</td>
<td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase Buffer</td>
+
<td>Ligase Buffer(μL)</td>
<td>4</td><td>4</td>
<td>4</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>4</td><td>4</td>
<td>4</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
<td>20</td><td>20</td>
<td>20</td><td>20</td>
</tr>
</tr>
</table>
</table>
-
<br>TRANSFORMATION
+
[[Team:Chiba/protocol/transformation|transformation]]
-
<br>BBa_J54103(RBS+GFP)
+
*[http://partsregistry.org/Part:BBa_J54103 BBa_J54103](RBS+GFP)
-
<br>BBa_P2006
+
*[http://partsregistry.org/Part:BBa_P2006 BBa_P2006]
[[Team:Chiba/protocol/gelcheck|Gel check]]
[[Team:Chiba/protocol/gelcheck|Gel check]]
-
<br>Ligation product(Venus YFP)
+
*Ligation product(Venus YFP)
-
<br>BBa_J37033
+
*BBa_J37033
-
<br>BBa_J37020
+
*BBa_J37020
-
<br>BBa_J37032
+
*BBa_J37032
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>

Revision as of 03:37, 25 October 2008

>Home | Notebook

17 September 2008 <|> 19 September 2008

Laboratory work

Team:Communication

  1. strain
    Senders:XL10GOLD
    Receiver:JW1908
  2. temparature
    37℃ and 30℃


Team:Output

SAP 1μL

->left for:

  • 1 hour at 37 degrees
  • 30 minutes at 65 degrees.

Zymoclean

->eluted with 25μL of NFW.

Ligation

  • vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
  • insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
  • Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
Sample No. Vector&insertVector
DNA(vector)(μL) 3.53.5
DNA(insert)(μL) 7.5-
Ligase(μL) 11
Ligase Buffer(μL) 44
dH2O(μL) 44
TOTAL(μL) 2020

transformation

  • [http://partsregistry.org/Part:BBa_J54103 BBa_J54103](RBS+GFP)
  • [http://partsregistry.org/Part:BBa_P2006 BBa_P2006]

Gel check

  • Ligation product(Venus YFP)
  • BBa_J37033
  • BBa_J37020
  • BBa_J37032
Sample No each
DNA each
Loading Dye 1
dH2O 4
TOTAL 6
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