Chiba/Sender experiments

From 2008.igem.org

(Difference between revisions)
(New page: ===Quorum-Sensing Cross-talk=== *'''Senders''' **[http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI] Image:Tet-lasi chiba.gif **[http://partsregistry.org/Part:BBa_K084008 plac+r...)
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[[Image:Chiba-U.gif]]
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{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
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!align="center"|[[Team:Chiba|Home]]
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!align="center"|[[Team:Chiba/Team|The Team]]
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!align="center"|[[Team:Chiba/Project|The Project]]
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!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
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|}
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<span style="font-size:120%;font-weight:bold;">[[Chiba/Project_Design|Project Design]] ( [[Chiba/Sender_experiments|Sender 実験]] | [[Chiba/Receiver_experiments|レシーバー実験]] )  [[Chiba/Making time_delay|Making time delay]] |  [[Chiba/Goal|Our Goal]]</span><br>
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===Quorum-Sensing Cross-talk===
===Quorum-Sensing Cross-talk===
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**[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
**[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
[[Image:Reporter 9002 chiba.gif]]
[[Image:Reporter 9002 chiba.gif]]
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===Method===
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To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
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#Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
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#Inoculated them independently in liquid media. Incubated at 37℃ 12h
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#Mixed them.
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#Incubated at 37℃ or 30℃.
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#Measured intensity of green fluorescence at regular time intervals.
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===Result===
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{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
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!align="center"|[[Team:Chiba|Home]]
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!align="center"|[[Team:Chiba/Team|The Team]]
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!align="center"|[[Team:Chiba/Project|The Project]]
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!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
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|}

Revision as of 04:53, 25 October 2008

Chiba-U.gif

Home The Team The Project Parts Submitted to the Registry Notebook

Project Design ( Sender 実験 | レシーバー実験 ) Making time delay | Our Goal


Quorum-Sensing Cross-talk

  • Senders
    • [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]

Tet-lasi chiba.gif

    • [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]

Tet-rhli chiba.gif

    • [http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]

Tet-luxi chiba.gif

  • Receiver
    • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

Reporter 9002 chiba.gif


Method

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

  1. Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37℃ 12h
  3. Mixed them.
  4. Incubated at 37℃ or 30℃.
  5. Measured intensity of green fluorescence at regular time intervals.

Result

Home The Team The Project Parts Submitted to the Registry Notebook
Retrieved from "http://2008.igem.org/Chiba/Sender_experiments"