Team:Chiba/Sender experiments

From 2008.igem.org

(Difference between revisions)
(→Method)
Line 11: Line 11:
{| style="border:0;width:100%;font-family:'Trebuchet MS'" cellpadding="20px" cellspacing="0"
{| style="border:0;width:100%;font-family:'Trebuchet MS'" cellpadding="20px" cellspacing="0"
| align="center" |
| align="center" |
-
<span style="font-size:120%;font-weight:bold;">[[Team:Chiba/Project|Project Design]] ( [[Team:Chiba/Sender_experiments|Sender 実験]] | [[Team:Chiba/Receiver_experiments|レシーバー実験]] )  [[Team:Chiba/Making time_delay|Making time delay]] |  [[Team:Chiba/Goal|Our Goal]]</span><br>
+
<span style="font-size:120%;font-weight:bold;">[[Team:Chiba/Project|Project Design]] ( [[Team:Chiba/Sender_experiments|Sender 実験]] | [[Team:Chiba/Receiver_experiments|レシーバー実験]] )  |  [[Team:Chiba/Goal|Our Goal]]</span><br>
|}
|}

Revision as of 05:04, 25 October 2008

Chiba-U.gif

Home The Team The Project Parts Submitted to the Registry Notebook

Project Design ( Sender 実験 | レシーバー実験 ) | Our Goal

Quorum-Sensing Cross-talk

  • Senders
    • [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]

Tet-lasi chiba.gif

    • [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]

Tet-rhli chiba.gif

    • [http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]

Tet-luxi chiba.gif

  • Receiver
    • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

Reporter 9002 chiba.gif


Method

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

  1. Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37°C 12h
  3. Mixed them.
  4. Incubated at 37°C or 30°C.
  5. Measured intensity of green fluorescence at regular time intervals.

Result

Home The Team The Project Parts Submitted to the Registry Notebook
Retrieved from "http://2008.igem.org/Team:Chiba/Sender_experiments"