Team:Chiba/Receiver experiments

From 2008.igem.org

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===LuxR/Plux mutants===
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==LuxR/Plux mutants==
===method===
===method===
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===result===
===result===
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===AiiA Receiver===
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==AiiA Receiver==
[[Image:AiiA-Receiver Chiba.gif|frame|right|'''Fig.6''' AiiA Receiver]]
[[Image:AiiA-Receiver Chiba.gif|frame|right|'''Fig.6''' AiiA Receiver]]

Revision as of 05:06, 25 October 2008

Chiba-U.gif

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Project Design ( Sender experiments | Receiver experiments ) | Our Goal

Contents

LuxR/Plux mutants

method

result

AiiA Receiver

Fig.6 AiiA Receiver

Design

オートインデューサー不活性化酵素であるAiiAをレシーバー菌で発現させることにより、レシーバー菌内でのAHL濃度の増加が遅くなるため、AiiAを発現しないレシーバー菌に比べて、GFP発現が遅くなる。

Experiment

  • Sender
    • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623 (AHL auto inucer)]

LuxI-sender Chiba.gif


method

result

  • Receivers
    • AiiA Receiver

AiiA-Receiver-genetic-circu Chiba.gif

    • non-AiiA Receiver

Non-AiiA-Receiver Chiba.gif


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