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Team:NTU-Singapore/Notebook/18 June 2008
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(New page: =Wednesday 18 June= These tasks are carried out for E7 insert and empty plasmid vector for RBS 0034: *4:30 pm: 1st digestion *6:30 pm: PCR purification, followed by 2nd digestion *11:30 pm...) |
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=Wednesday 18 June= | =Wednesday 18 June= | ||
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These tasks are carried out for E7 insert and empty plasmid vector for RBS 0034: | These tasks are carried out for E7 insert and empty plasmid vector for RBS 0034: | ||
*4:30 pm: 1st digestion | *4:30 pm: 1st digestion | ||
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*11:30 pm: collect digested product and freeze at -20 to prevent reaction. | *11:30 pm: collect digested product and freeze at -20 to prevent reaction. | ||
*Also, at 6:15pm, we plate LacI promoter in Homemade Top 10 and DH5α | *Also, at 6:15pm, we plate LacI promoter in Homemade Top 10 and DH5α | ||
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Revision as of 11:15, 25 October 2008
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Wednesday 18 June
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These tasks are carried out for E7 insert and empty plasmid vector for RBS 0034:
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