Team:NTU-Singapore/Notebook/27 July 2008

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(New page: Darius *Digested Lysis and terminator for an 1 and a half hour. ** did a variation of lysis and terminator between inserts and vectors. ***Gel extracted lysis insert(E & X)(sequential dige...)
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=Sunday 27 July=
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'''Darius'''
*Digested Lysis and terminator for an 1 and a half hour.
*Digested Lysis and terminator for an 1 and a half hour.
** did a variation of lysis and terminator between inserts and vectors.
** did a variation of lysis and terminator between inserts and vectors.
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* Fast ligation.
* Fast ligation.
*Transformation
*Transformation
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Revision as of 12:28, 25 October 2008

Sunday 27 July

Darius

  • Digested Lysis and terminator for an 1 and a half hour.
    • did a variation of lysis and terminator between inserts and vectors.
      • Gel extracted lysis insert(E & X)(sequential digestion, PCR purification)and lysis vector(E and S)( Non sequential). Gel extracted terminator insert(S & P) and terminator vector(E & X) (sequential).
  • Fast ligation.
  • Transformation