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Team:Warsaw/Calendar-Main/19 June 2008
From 2008.igem.org
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<li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br> | <li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br> | ||
| - | There is no mutation in beta-galactosidase gene. Why | + | There is no mutation in beta-galactosidase gene. Why are the colonies white?</li> |
<li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br> | <li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br> | ||
Revision as of 12:32, 25 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin). Blue/white testPiotrThere is no mutation in beta-galactosidase gene. Why are the colonies white? Template: DNA isolated from white colonies Primers: pZCseqL and pZCseqR
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