Team:Warsaw/Calendar-Main/10 October 2008

(Difference between revisions)

Revision as of 14:45, 25 October 2008


	Attribution analyzer / Interactive list of topics

Visas have been accorded to the whole team.

Digest of RFP(?????)+deltaA with EcoRI and SacI (BamHI buffer), dephosphorylation with CIAP
Gel electrophoresis and gel-out of proper band 2200 bp.
Ligation of digested 5kb (??????) vector from (30 September) with pT7_omega_link and RFP(?????) with pT7_alpha fragment (1 hr).

@@ Line 4: / Line 4: @@
 <h3>Visit in US Embassy</h3>
 <p>Visas have been accorded to the whole team.</p>
+<h3>Preparation of BioBricks</h3>
+<h4>Michał K.</h4>
+<ol>
+<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>Digest</a> of
+RFP(?????)+deltaA with EcoRI and SacI (BamHI buffer), <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation</a> with CIAP </li>
+<li>Gel electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band 2200 bp. </li>
+<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested  5kb (??????) vector from (30 September) with pT7_omega_link and  RFP(?????) with pT7_alpha fragment (1 hr).</li>
+<h3></h3>
+<h4>Piotr</h4>
+<ol><h3></h3>
+<h4>Piotr</h4>
+<ol>
+<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligations.</li>
+<li> Tranformants planting on LB with kanamycin. </li>
 </html>