Team:Warsaw/Calendar-Main/20 October 2008

From 2008.igem.org

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<li> Inoculation of colonies from plate with ligation of 5kb(?????) + pT7_alpha to LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin. </li>
<li> Inoculation of colonies from plate with ligation of 5kb(?????) + pT7_alpha to LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin. </li>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>Digest</a> of
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3kb(????)+pLac_OmpA_alpha and RFP(????)+OmpA  with EcoRI and SacI (BamHI buffer). </li>
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<li>Gel electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band 500 bp for RFP(????)+OmpA and 3000 bp for 3kb(????)+pLac_OmpA_alpha. </li>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of DNA fragments: 3kb(?????)_ alpha + Omp.</li></ol>
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<h4>Piotr</h4>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligation.</li>
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<li> Tranformants planting on LB with kanamycin. </li>
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Revision as of 21:38, 25 October 2008

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Preparation of BioBricks

Michał K.

  1. Inoculation of colonies from plate with ligation of 5kb(?????) + pT7_alpha to LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin.
  2. Digest of 3kb(????)+pLac_OmpA_alpha and RFP(????)+OmpA with EcoRI and SacI (BamHI buffer).
  3. Gel electrophoresis and gel-out of proper band 500 bp for RFP(????)+OmpA and 3000 bp for 3kb(????)+pLac_OmpA_alpha.
  4. Ligation of DNA fragments: 3kb(?????)_ alpha + Omp.

Piotr

  1. Transformation of TOP10 with above ligation.
  2. Tranformants planting on LB with kanamycin.

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