Team:Warsaw/Calendar-Main/7 May 2008

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<h3>Preparation of <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID">pMPMT5+AID</a> construct</h3>
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<h4>Piotr</h4>
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Piotr: <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids DNA</a>: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> (the last one from 15 ml of culture because it's one-copy plasmid)</p>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmid DNA</a>: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> (the last one from 15 ml of culture because it's one-copy plasmid).</p>
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Preparation of pMPMT5+AID construct

Piotr

Isolation of plasmid DNA: pTrc99A-AID, pMPM-T5, pZC320 (the last one from 15 ml of culture because it's one-copy plasmid).