Team:UNIPV-Pavia/Parts

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Revision as of 00:31, 26 October 2008

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Assembled Parts Notation

In notebook section we sometimes use abbreviations to name assembled parts. New parts' official name, notebook abbreviations and new parts description are reported in the following table:

New Part	Notebook Name	Assembled Parts(code)	Assembled Parts(name)	Vector	Description
-	01	J23100-E0240	Pcon-GFP_protein_generator	J61002	Test
-	02	J23100-B0030	Pcon-RBS	pSB1A2	Intermediate
-	03	R0051-B0030	Plambda-RBS	pSB1A2	Intermediate
-	04	B0030-E0040	RBS-GFP	pSB1A2	Intermediate
-	05	B0030-C0051	RBS-cI	pSB1A2	Intermediate
-	06	23100-E1010	RBS-RFP	pSB1A2	Intermediate
-	07	B0030-C0061	RBS-luxI	pSB1A2	Intermediate
-	08	B0030-C0078	RBS-lasI	pSB1A2	Intermediate
-	09	J23100-B0030-C0012	Pcon-RBS-lacI	pSB1A2	Intermediate
-	10	J23100-B0030-C0040	Pcon-RBS-tetR	pSB1A2	Intermediate
-	11	J23100-B0030-I15010	Pcon-RBS-cph8	pSB1A2	Intermediate
-	12	R0051-B0030-C0062	Plambda-RBS-luxR	pSB1A2	Intermediate
-	13	B0030-E0040-B1006	RBS-GFP-T	pSB1AK3	GFP protein generator with 39bp artificial terminator
-	14	B0030-C0051-B0030	RBS-cI-RBS	pSB1A2	Intermediate
-	15	B0030-E1010-B1006	RBS-RFP-T	pSB1AK3	GFP protein generator with 39bp artificial terminator
-	16	B0030-C0061-B1006	RBS-luxI-T	pSB1AK3	luxI protein generator RCT
-	17	B0030-C0078-B1006	RBS-lasI-T	pSB1AK3	lasI protein generator RCT
-	18	B0030-I15009	RBS-pcyA	pSB1A2	Intermediate
-	19	J23100-B0030-C0012-B1006	Pcon-RBS-lacI-T	pSB1AK3	lacI protein generator PRCT
-	20	J23100-B0030-C0040-B1006	Pcon-RBS-tetR-T	pSB1AK3	tetR protein generator PCRT
-	21	J23100-B0030-I15010-B0030	Pcon-RBS-cph8-RBS	?	Intermediate
-	22	R0051-B0030-C0062-B1006	Plambda-RBS-luxR-T	pSB1AK3	Plambda regulated luxR protein generator PRCT
-	23	B0030-C0051-B0030-C0079	RBS-cI-RBS-lasR	pSB1A2	Intermediate
-	24	B0030-C0061-B1006-R0062	RBS-luxI-T-Plux	pSB1A2	Mux - Channel 0
-	25	B0030-C0078-B1006-R0079	RBS-lasI-T-Plas	pSB1A2	Mux - Channel 1
-	26	B0030-I15009-B1006	RBS-pcyA-T	pSB1AK3	pcyA protein generator RCT
-	27	J23100-B0030-C0012-B1006-R0010	Pcon-RBS-lacI-T-Plac	pSB1AK3	lacI composite part: PRCT-Plac
-	28	J23100-B0030-C0040-B1006-R0040	Pcon-RBS-tetR-T-Ptet	pSB1A2	tetR composite part: PRCT-Ptet
-	29	J23100-B0030-I15010-B0030-I15008	Pcon-RBS-cph8-RBS-ho1	?	Intermediate
-	30	R0051-B0030-C0062-B1006-R0062	Plambda-RBS-luxR-T-Plux	pSB1A2	luxR composite part: Plambda regulated PRCT-Plux
-	31	B0030-C0051-B0030-C0079-B1006	RBS-cI-RBS-lasR-T	pSB1AK3	cI-lasR protein generator RCRCT
-	32	B0030-I15009-B1006-R0082	RBS-pcyA-T-Pomp	pSB1A2	pcyA composite part: RCT-Pomp
-	33	J23100-B0030-I15010-B0030-I15008-B0030-I15009-B1006-R0082	Pcon-RBS-cph8-RBS-ho1-RBS-pcyA-T-Pomp	?	Light responsive system under constitutive promoter: PRCRCRCT-Pomp
-	34	B0030-C0051-B0030-C0079-B1006-R0051-B0030-C0062-B1006-R0062	RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T-Plux	pSB1AK3	Demux - Selector
-	35	B0030-C0051-B0030-C0079-B1006-R0051-B0030-C0062-B1006	RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T	pSB1AK3	Mux - Selector
-	36	B0030-C0061-B0030-C0078-B1006-R0079	RBS-luxI-RBS-lasI-T-Plas	pSB1A2	Demux - Input

Red-highlighted parts (11, 21, 29, 33) contain I15010 (cph8): according to IGEM DNA Repositories Quality Control (and according to our results) the sequence is inconsistent and so these parts cannot be assembled.
Green-coloured parts have been succesfully sequenced.
24, 25, 34, 35, 36 are part of our final Mux or Demux systems.
Parts in gray have not been assembled yet (updated: 07-29-2008).

@@ Line 31: / Line 31: @@
 |''-''
 |01
-|BBa_J23100-BBa_E0240
+|J23100-E0240
 |Pcon-GFP_protein_generator
-|BBa_J61002
+|J61002
 |Test
 |-
 |<font color="green">''-''</font>
 |<font color="green">'''02'''</font>
-|<font color="green">'''BBa_J23100-BBa_B0030'''</font>
+|<font color="green">'''J23100-B0030'''</font>
 |<font color="green">'''Pcon-RBS'''</font>
 |<font color="green">'''pSB1A2'''</font>
@@ Line 45: / Line 45: @@
 |<font color="green">''-''</font>
 |<font color="green">'''03'''</font>
-|<font color="green">'''BBa_R0051-BBa_B0030'''</font>
+|<font color="green">'''R0051-B0030'''</font>
 |<font color="green">'''Plambda-RBS'''</font>
 |<font color="green">'''pSB1A2'''</font>
@@ Line 52: / Line 52: @@
 |<font color="green">'''-'''</font>
 |<font color="green">'''04'''</font>
-|<font color="green">'''BBa_B0030-BBa_E0040'''</font>
+|<font color="green">'''B0030-E0040'''</font>
 |<font color="green">'''RBS-GFP'''</font>
 |<font color="green">'''pSB1A2'''</font>
@@ Line 59: / Line 59: @@
 |<font color="green">'''-'''</font>
 |<font color="green">'''05'''</font>
-|<font color="green">'''BBa_B0030-BBa_C0051'''</font>
+|<font color="green">'''B0030-C0051'''</font>
 |<font color="green">'''RBS-cI'''</font>
 |<font color="green">'''pSB1A2'''</font>
@@ Line 66: / Line 66: @@
 |<font color="green">'''-'''</font>
 |<font color="green">'''06'''</font>
-|<font color="green">'''BBa_J23100-BBa_E1010'''</font>
+|<font color="green">'''23100-E1010'''</font>
 |<font color="green">'''RBS-RFP'''</font>
 |<font color="green">'''pSB1A2'''</font>
@@ Line 73: / Line 73: @@
 |<font color="green">'''-'''</font>
 |<font color="green">'''07'''</font>
-|<font color="green">'''BBa_B0030-BBa_C0061'''</font>
+|<font color="green">'''B0030-C0061'''</font>
 |<font color="green">'''RBS-luxI'''</font>
 |<font color="green">'''pSB1A2'''</font>
@@ Line 80: / Line 80: @@
 |<font color="green">'''-'''</font>
 |<font color="green">'''08'''</font>
-|<font color="green">'''BBa_B0030-BBa_C0078'''</font>
+|<font color="green">'''B0030-C0078'''</font>
 |<font color="green">'''RBS-lasI'''</font>
 |<font color="green">'''pSB1A2'''</font>
@@ Line 87: / Line 87: @@
 |style="background:gray"|''-''
 |style="background:gray"|09
-|style="background:gray"|BBa_J23100-BBa_B0030-BBa_C0012
+|style="background:gray"|J23100-B0030-C0012
 |style="background:gray"|Pcon-RBS-lacI
 |style="background:gray"|pSB1A2
@@ Line 94: / Line 94: @@
 |''-''
 |10
-|BBa_J23100-BBa_B0030-BBa_C0040
+|J23100-B0030-C0040
 |Pcon-RBS-tetR
 |pSB1A2
@@ Line 101: / Line 101: @@
 |style="background:red; color:white"|''-''
 |style="background:red; color:white"|11
-|style="background:red; color:white"|BBa_J23100-BBa_B0030-BBa_I15010
+|style="background:red; color:white"|J23100-B0030-I15010
 |style="background:red; color:white"|Pcon-RBS-cph8
 |style="background:red; color:white"|pSB1A2
@@ Line 108: / Line 108: @@
 |''-''
 |12
-|BBa_R0051-BBa_B0030-BBa_C0062
+|R0051-B0030-C0062
 |Plambda-RBS-luxR
 |pSB1A2
@@ Line 115: / Line 115: @@
 |<font color="green">'''-'''</font>
 |<font color="green">'''13'''</font>
-|<font color="green">'''BBa_B0030-BBa_E0040-BBa_B1006'''</font>
+|<font color="green">'''B0030-E0040-B1006'''</font>
 |<font color="green">'''RBS-GFP-T'''</font>
 |<font color="green">'''pSB1AK3'''</font>
@@ Line 122: / Line 122: @@
 |''-''
 |14
-|BBa_B0030-BBa_C0051-BBa_B0030
+|B0030-C0051-B0030
 |RBS-cI-RBS
 |pSB1A2
@@ Line 129: / Line 129: @@
 |<font color="green">'''-'''</font>
 |<font color="green">'''15'''</font>
-|<font color="green">'''BBa_B0030-BBa_E1010-BBa_B1006'''</font>
+|<font color="green">'''B0030-E1010-B1006'''</font>
 |<font color="green">'''RBS-RFP-T'''</font>
 |<font color="green">'''pSB1AK3'''</font>
@@ Line 136: / Line 136: @@
 |''-''
 |16
-|BBa_B0030-BBa_C0061-BBa_B1006
+|B0030-C0061-B1006
 |RBS-luxI-T
 |pSB1AK3
@@ Line 143: / Line 143: @@
 |''-''
 |17
-|BBa_B0030-BBa_C0078-BBa_B1006
+|B0030-C0078-B1006
 |RBS-lasI-T
 |pSB1AK3
@@ Line 150: / Line 150: @@
 |''-''
 |18
-|BBa_B0030-BBa_I15009
+|B0030-I15009
 |RBS-pcyA
 |pSB1A2
@@ Line 157: / Line 157: @@
 |style="background:gray"|''-''
 |style="background:gray"|19
-|style="background:gray"|BBa_J23100-BBa_B0030-BBa_C0012-BBa_B1006
+|style="background:gray"|J23100-B0030-C0012-B1006
 |style="background:gray"|Pcon-RBS-lacI-T
 |style="background:gray"|pSB1AK3
@@ Line 164: / Line 164: @@
 |''-''
 |20
-|BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006
+|J23100-B0030-C0040-B1006
 |Pcon-RBS-tetR-T
 |pSB1AK3
@@ Line 171: / Line 171: @@
 |style="background:red; color:white"|''-''
 |style="background:red; color:white"|21
-|style="background:red; color:white"|BBa_J23100-BBa_B0030-BBa_I15010-BBa_B0030
+|style="background:red; color:white"|J23100-B0030-I15010-B0030
 |style="background:red; color:white"|Pcon-RBS-cph8-RBS
 |style="background:red; color:white"|?
@@ Line 178: / Line 178: @@
 |''-''
 |22
-|BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006
+|R0051-B0030-C0062-B1006
 |Plambda-RBS-luxR-T
 |pSB1AK3
@@ Line 185: / Line 185: @@
 |''-''
 |23
-|BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079
+|B0030-C0051-B0030-C0079
 |RBS-cI-RBS-lasR
 |pSB1A2
@@ Line 192: / Line 192: @@
 |<font color="green">'''-'''</font>
 |<font color="green">'''24'''</font>
-|<font color="green">'''BBa_B0030-BBa_C0061-BBa_B1006-BBa_R0062'''</font>
+|<font color="green">'''B0030-C0061-B1006-R0062'''</font>
 |<font color="green">'''RBS-luxI-T-Plux'''</font>
 |<font color="green">'''pSB1A2'''</font>
@@ Line 199: / Line 199: @@
 |<font color="green">'''-'''</font>
 |<font color="green">'''25'''</font>
-|<font color="green">'''BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079'''</font>
+|<font color="green">'''B0030-C0078-B1006-R0079'''</font>
 |<font color="green">'''RBS-lasI-T-Plas'''</font>
 |<font color="green">'''pSB1A2'''</font>
@@ Line 206: / Line 206: @@
 |''-''
 |26
-|BBa_B0030-BBa_I15009-BBa_B1006
+|B0030-I15009-B1006
 |RBS-pcyA-T
 |pSB1AK3
@@ Line 213: / Line 213: @@
 |style="background:gray"|''-''
 |style="background:gray"|27
-|style="background:gray"|BBa_J23100-BBa_B0030-BBa_C0012-BBa_B1006-BBa_R0010
+|style="background:gray"|J23100-B0030-C0012-B1006-R0010
 |style="background:gray"|Pcon-RBS-lacI-T-Plac
 |style="background:gray"|pSB1AK3
@@ Line 220: / Line 220: @@
 |style="background:gray"|''-''
 |style="background:gray"|28
-|style="background:gray"|BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040
+|style="background:gray"|J23100-B0030-C0040-B1006-R0040
 |style="background:gray"|Pcon-RBS-tetR-T-Ptet
 |style="background:gray"|pSB1A2
@@ Line 227: / Line 227: @@
 |style="background:red; color:white"|''-''
 |style="background:red; color:white"|29
-|style="background:red; color:white"|BBa_J23100-BBa_B0030-BBa_I15010-BBa_B0030-BBa_I15008
+|style="background:red; color:white"|J23100-B0030-I15010-B0030-I15008
 |style="background:red; color:white"|Pcon-RBS-cph8-RBS-ho1
 |style="background:red; color:white"|?
@@ Line 234: / Line 234: @@
 |''-''
 |30
-|BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-BBa_R0062
+|R0051-B0030-C0062-B1006-R0062
 |Plambda-RBS-luxR-T-Plux
 |pSB1A2
@@ Line 241: / Line 241: @@
 |''-''
 |31
-|BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006
+|B0030-C0051-B0030-C0079-B1006
 |RBS-cI-RBS-lasR-T
 |pSB1AK3
@@ Line 248: / Line 248: @@
 |''-''
 |32
-|BBa_B0030-BBa_I15009-BBa_B1006-BBa_R0082
+|B0030-I15009-B1006-R0082
 |RBS-pcyA-T-Pomp
 |pSB1A2
@@ Line 255: / Line 255: @@
 |style="background:red; color:white"|''-''
 |style="background:red; color:white"|33
-|style="background:red; color:white"|BBa_J23100-BBa_B0030-BBa_I15010-BBa_B0030-BBa_I15008-BBa_B0030-BBa_I15009-BBa_B1006-BBa_R0082
+|style="background:red; color:white"|J23100-B0030-I15010-B0030-I15008-B0030-I15009-B1006-R0082
 |style="background:red; color:white"|Pcon-RBS-cph8-RBS-ho1-RBS-pcyA-T-Pomp
 |style="background:red; color:white"|?
@@ Line 262: / Line 262: @@
 |'''-'''
 |'''34'''
-|'''BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-BBa_R0062'''
+|'''B0030-C0051-B0030-C0079-B1006-R0051-B0030-C0062-B1006-R0062'''
 |'''RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T-Plux'''
 |'''pSB1AK3'''
@@ Line 269: / Line 269: @@
 |'''-'''
 |'''35'''
-|'''BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006'''
+|'''B0030-C0051-B0030-C0079-B1006-R0051-B0030-C0062-B1006'''
 |'''RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T'''
 |'''pSB1AK3'''
@@ Line 276: / Line 276: @@
 |'''-'''
 |'''36'''
-|'''BBa_B0030-BBa_C0061-BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079'''
+|'''B0030-C0061-B0030-C0078-B1006-R0079'''
 |'''RBS-luxI-RBS-lasI-T-Plas'''
 |'''pSB1A2'''
@@ Line 283: / Line 283: @@
 <br>
-*<font color="red">'''Red'''</font>-highlighted parts (11, 21, 29, 33) contain BBa_I15010 (cph8): according to IGEM DNA Repositories Quality Control (and according to our results) the sequence is inconsistent and so these parts cannot be assembled.
+*<font color="red">'''Red'''</font>-highlighted parts (11, 21, 29, 33) contain I15010 (cph8): according to IGEM DNA Repositories Quality Control (and according to our results) the sequence is inconsistent and so these parts cannot be assembled.
 *<font color="green">'''Green'''</font>-coloured parts have been succesfully sequenced.
 *24, 25, 34, 35, 36 are part of our final Mux or Demux systems.
 *Parts in <font color="gray">'''gray'''</font> have not been assembled yet (updated: 07-29-2008).