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Team:Tokyo Tech/Construction
From 2008.igem.org
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For confirming pressure-response ability of pressure-inducible promoter, we | For confirming pressure-response ability of pressure-inducible promoter, we | ||
| - | experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part: | + | experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6(http://partsregistry.org/Part:BBa_K121013 BBa_K121013). |
Revision as of 06:04, 26 October 2008
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Construction
For confirming pressure-response ability of pressure-inducible promoter, we experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6(http://partsregistry.org/Part:BBa_K121013 BBa_K121013).
