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Team:Tokyo Tech/Construction
From 2008.igem.org
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[[Image:Construction.png|260px|thumb|left|figure1 We constructed A.PtetR-GFP, B.promoter less-GFP for confirmatory experiment]] | [[Image:Construction.png|260px|thumb|left|figure1 We constructed A.PtetR-GFP, B.promoter less-GFP for confirmatory experiment]] | ||
| - | + | We experimented under several pressures so as to confirm pressure-response ability of TetR promoter(PtetR) which we chose as pressure-inducible promoter. | |
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we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]). | we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]). | ||
Revision as of 07:08, 26 October 2008
| Home | Construction | Acrylic container | Development of promoter | Genetic toggle switch | Parts Submitted to the Registry | Result | The Team | Notebook |
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Construction
We experimented under several pressures so as to confirm pressure-response ability of TetR promoter(PtetR) which we chose as pressure-inducible promoter. we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]).
