Revision as of 09:45, 26 October 2008

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Welcome to iGEM08 Tsinghua's wiki page

Intro of Our Project

Tsinghua University was established in 1911, originally under the name “Tsinghua Xuetang”. The school was renamed "Tsinghua School" in 1912. The university section was founded in 1925. The name “National Tsinghua University” was adopted in 1928.click here to see more pictures

The educational philosophy of Tsinghua is to "train students with integrity." Among over 120,000 students who have graduated from Tsinghua since its founding are many outstanding scholars, eminent entrepreneurs and great statesmen remembered and respected by their fellow Chinese citizens.

With the motto of “Self-Discipline and Social Commitment” and the spirit of “Actions Speak Louder than Words”, Tsinghua University is dedicated to the well-being of Chinese society and to world development.

News

GF taq is recommended in our PCR reactions. 2008.June.25

The first plasmid is successfully constrcuted. 2008.Jul.20

The gene we need is at synthesis in Geneart. 2008.Jul.24

The standard vector pSB1A3 lost in the transformation. 2008.Jul.28

Then it finally succeeds. 2008.Jul.31

Template of one of the receptors are still in vacancy.

New edition of project interpretation are in process.

Intro of Tsinghua University

Tsinghua University was established in 1911, originally under the name “Tsinghua Xuetang”. The school was renamed "Tsinghua School" in 1912. The university section was founded in 1925. The name “National Tsinghua University” was adopted in 1928.click here to see more pictures

The educational philosophy of Tsinghua is to "train students with integrity." Among over 120,000 students who have graduated from Tsinghua since its founding are many outstanding scholars, eminent entrepreneurs and great statesmen remembered and respected by their fellow Chinese citizens.

With the motto of “Self-Discipline and Social Commitment” and the spirit of “Actions Speak Louder than Words”, Tsinghua University is dedicated to the well-being of Chinese society and to world development.

== Our Schedule ==

  Time	                      Events
Dec. 15, 2007	              Team of Tsinghua University Founded
Dec. 31, 2007	              Brain Storm(1)
Jan-Feb, 2008	              Backgroud Investigation
Feb.25, 2008	              Brain Storm(2)
Mar., 2008	              Experimental procedure design;
                             Modeling in silico Started  
Apr.1,2008	              Wet Lab work started
Apr.-Aug.,2008	              Wet Lab Work
May.1, 2008	              Team Registry
May.25, 2008	              Students’ Association of Synthetic Biology(SASB) Founded
June.21, 2008	              “Teach the teacher “workshop, in Kyoto, Japan
Sept.-Oct., 2008	      Inspection and Revision     
Oct.20,2008	              Summary, Presentation Preparation
Nov.8-9,2008	              Jamboree In MIT

Molecular cloning

  1   PCR
    
 1.1 Gold Faster 	Taq system:
   Reagent	           Concentration/Activity	50ul 	100ul
   GF taq buffer     	    10x	                         5       10
   GF taq		                                 0.5	  0.5
   dNTPmix	            10mM each	                 1	  2
   Primer 1 	            10uM	                 1	  2
   Primer 2	            10um	                 1	  2
   Template DNA	      changeable	                 0.5	  1
   MgCl2	            0.2M	                 0.5	  1
   ddH2O	                ---	                40.5	 81
 1.2 The program under Gold faster taq system
   Progress	     Program I	                 Program 2
   Predenaturing    95℃         2-5 min	         95℃   2-5 min
   Denaturing	     95℃        10-20sec	         95℃   10-20sec
   Annealing	     (Tm-5) ℃   2-5 sec	         68℃   10-15sec/1kb
   Extension	     72℃        10-15sec/1kb

25-30cycle 25-30cycle

   Last extension	  1-2min or skipped	       1-2min or skipped

   2 	Restriction cut:
           Reagent	        Concentration/Activity	 Volume(50ul system)
           Restriction cut buffer	10x	           5ul
           Enzyme 1	                --	           2.5ul
           Enzyme 2	                --	           2.5ul
 Incubate at 37℃, 1.5 hrs or longer
 (Enzymes from Takara Co., Ltd)

     3  Ligation:
              Reagent	    Volume(10ul system)
              Solution I	5ul
              DNA fragment	3.5ul
              Vector	        1.5ul
   Incubate at 16-18℃,1hr or longer
   (Ligation kit from Takara)

Home	Team	Project	Parts	Modelling	Notebook	Doodle Board

@@ Line 16: / Line 16: @@
 {|align="justify"
-|our project
+|Tsinghua University was established in 1911, originally under the name “Tsinghua Xuetang”. The school was renamed "Tsinghua School" in 1912. The university section was founded in 1925. The name “National Tsinghua University” was adopted in 1928.[[gallery|click here to see more pictures]]
+The educational philosophy of Tsinghua is to "train students with integrity." Among over 120,000 students who have graduated from Tsinghua since its founding are many outstanding scholars, eminent entrepreneurs and great statesmen remembered and respected by their fellow Chinese citizens.
+With the motto of “Self-Discipline and Social Commitment” and the spirit of “Actions Speak Louder than Words”, Tsinghua University is dedicated to the well-being of Chinese society and to world development.
 |[[image:Thu_Logo.jpg|right]]
 |}
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   Oct.20,2008	              Summary, Presentation Preparation
   Nov.8-9,2008	              Jamboree In MIT
-== == Abstract == ==
-   This year we are trying to work at the motility of bacteria. E.coli senses some chemicals and moves toward
-or stays still, this behavior is called chemotaxis. Any modification of the pathway from the sensor to
-the        motile apparatus will cause into different chemotactic effcts.
-  Moreover，most of the biosensors today are designed for a specific target signal, and they can not
-substitute   each other in most cases. By structural analysis we find that most receptors for the same type
-of    ligands exhibits great similarity, both in sequence information and characteristics. Our project
-is         designed toward an "Interchangeable Biosensor". The strategy is almost trial and error, to find
-an     appropriate pathway between chemical molecules and corresponding reactions. Signals other than
-chemicals   are much harder to manipulate, and to integrate together the signals seems even more
-challenging,we    are trying to isolate the elements and standardise them.
-== == Background == ==
-Motile bacteria can respond to chemical gradients in their environment. Such directed movement in response to special chemicals is defined as chemotaxis. If organisms detect substances beneficial for surviving, positive chemotaxis occurs and cells will move in the direction of increasing concentration. In contrast, negative chemotaxis occurs when motile bacteria move away from a repellant. Both kinds of responses adjust the behaviors of organisms to the environment, thus improve their ability of surviving.
-One typical chemotactic behavior is the response of Escherichia coli toward certain nutritions, which plays a key role in seeking food. Researchers have investigated thoroughly into the molecular mechanism of it.
-Motility in prokaryotes is accomplished by flagellum, a complex structure moving the cell by rotation, much like the propeller in a motor boat. In E.coli, counterclockwise rotation moves the cell in a direction called a run, while clockwise rotation results in tumbling of the cell.
-The direction of rotation is controlled by phosphorylation of CheY, a signaling protein in the chemotaxis pathway of E.coli. Low level of phosphorylation results in counterclockwise rotation of flagella and makes the cell move forward. In contrast, phosphorylated CheY favors tumbling.
-The level of protein phosphorylation is regulated by certain attractant or repellent, which can bind specific receptors on the cell surface. Signals are then passed through a series of intracellular biochemical reactions, in which phosphorylation of CheY is controlled. In this way bacteria accomplish chemotactic behavior.
-Escherichia coli is a common kind of engineer bacteria, which is convenient for genetic manipulation. It is likely that we make use of the chemotactic pathway in E.coli, making them detect and move toward a special chemical substance, maybe a certain kind of pollutant. If the genetic engineering strain also has the ability of degrading aimed chemicals, efficiency of pollution treatment can be hopefully improved a lot. However, the natural ligands concerning chemotaxis in E.coli are limited in several kinds of nutrition, such as carbohydrate and amino acids. Strains that can detecting more kinds of organic substances and even heavy metal ions is planed to be got through the reconstruction of chemotaxis system.
 == Molecular cloning ==
@@ Line 132: / Line 109: @@
      (Ligation kit from Takara)
-== == Meet up == ==
-.Dec.1          With Peking University team leaders.
-.Dec.15         With all the students from different academies.
-. Feb           With candidate instructors in Our Department.
-. March         With Faculties from Professor Lee's lab.
-. April         With Drew Endy in Peking University.
-. April         With Peking University.
-. June.21-22    On Teachers' Workshop, with most Asia teams.
-. July.17       With Beijing Normal University Team
-.July.25        With Invitrogen.Co. Ltd.

Team:Tsinghua

From 2008.igem.org