Team:Warsaw/Calendar-Main/28 August 2008

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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li>  
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li>  
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<li>Gel electrophoresis.</li>
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<li>Gel electrophoresis (Fig. 1.).</li>
<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin.
<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin.
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<img src="https://static.igem.org/mediawiki/2008/8/8a/Kolos.jpg" width=300/> <var><b>Fig. 1. PCR on colonies from plates with transformation/b><br>
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1. Marker<br>
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2-23. PCR products<br></var>
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Revision as of 10:15, 26 October 2008

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Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Michał K.

  1. Colony PCR on colonies from plates with transformation pET15b+A_alpha.
    Primers used: AP+NotI and AL+SacI.
  2. Gel electrophoresis (Fig. 1.).
  3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.
Fig. 1. PCR on colonies from plates with transformation/b>
1. Marker
2-23. PCR products

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