Team:Tokyo Tech/Acrylic container

From 2008.igem.org

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== '''2. Acrylic container''' ==
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="85%" align="center"
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!align="center"|[[Team:Tokyo_Tech|Home]]
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!align="center"|[[Team:Tokyo_Tech/Construction|Construction]]
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!align="center"|[[Team:Tokyo_Tech/Acrylic container|Acrylic container]]
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!align="center"|[[Team:Tokyo_Tech/Development of low pressure inducible promoter|Development of promoter]]
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!align="center"|[[Team:Tokyo_Tech/Genetic toggle switch to implement rewritable function|Genetic toggle switch ]]
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!align="center"|[[Team:Tokyo_Tech/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Tokyo_Tech/Result|Result]]
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!align="center"|[[Team:Tokyo_Tech/Team|The Team]]
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!align="center"|[[Team:Tokyo_Tech/Acknowledgements|Acknowledgements]]
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|}
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== '''Acrylic container''' ==
<html>
<html>
  <body>
  <body>
   <div class=Section1 style='layout-grid:18.0pt'>
   <div class=Section1 style='layout-grid:18.0pt'>
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<table width="100%" border="0" cellspacing="0" cellpadding="0">
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        <tr>
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          <td class="a" width="20%">　</td>
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          <td class="a" width="50%">
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<IMG src="https://static.igem.org/mediawiki/2008/e/e6/Tech_aqulyl2.JPG
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" align="middle"></td>
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<td class="a" width="40%"></td>
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        </tr>
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      </table>
<table width="100%" border="0" cellspacing="0" cellpadding="0">
<table width="100%" border="0" cellspacing="0" cellpadding="0">
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           <td class="a" width="2%">　</td>
           <td class="a" width="2%">　</td>
           <td class="a">
           <td class="a">
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<p class=MsoNormal>We create devices for confirming pressure response of lac promoter. This is the first step of creating touch display. This device made of Acrylic glasses and has two holes (show figure). Each hole contains tubes and water. Inside tubes E. coli is cultivated. Pressure can travel to inside tubes. One hole (B) is covered with a block made of an acrylic glass. (show figure) Therefore the hole is not pressurized by water. The other (A) is covered with a plastic tape (show figure). Therefore the hole is pressurized. </font></p>
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<p class=MsoNormal>We create devices for confirming pressure response of lac promoter. This is the first step of creating touch display. This device made of Acrylic glasses and has two holes (show figure). Each hole contains tubes and water. Inside tubes E. coli is cultivated. Pressure can travel to inside tubes. One hole (A) is covered with a plastic tape (show figure). Therefore the hole is pressurized.The other (B) is covered with a block made of an acrylic glass. (show figure) Therefore the hole is not pressurized by water.   </font></p>
<td class="a" width="3%">　</td>
<td class="a" width="3%">　</td>
         </tr>
         </tr>
       </table>
       </table>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
<table width="100%" border="0" cellspacing="0" cellpadding="0">
<table width="100%" border="0" cellspacing="0" cellpadding="0">
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<table width="100%" border="0" cellspacing="0" cellpadding="0">
<table width="100%" border="0" cellspacing="0" cellpadding="0">
         <tr>  
         <tr>  
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           <td class="a" width="2%">　</td>
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          <td class="a" width="10%">　</td>
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           <td class="a" width="2%">　<IMG src="https://static.igem.org/mediawiki/2008/a/a5/Tech_aqulyl1.jpg
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" align="middle"></td>
           <td class="a">
           <td class="a">
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<p class=MsoNormal>* “Plac” stands for a promoter repressed by lac protein.
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<p class=MsoNormal>* “Ptet” on pSB6 plasmid (E.coli strain; JM109)</i></p></td>
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On pSB6 plasmid (E.coli strain; JM109)</i></p></td>
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<td class="a" width="3%">　</td>
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         </tr>
         </tr>
       </table>
       </table>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p><p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
<table width="100%" border="0" cellspacing="0" cellpadding="0">
<table width="100%" border="0" cellspacing="0" cellpadding="0">
         <tr>  
         <tr>  
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           <td class="a" width="3%">　</td>
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           <td class="a" width="2%">　</td>
           <td class="a">
           <td class="a">
<p class=MsoNormal>After pressurized the container, we observed the E.coli by a fluorescence microscope. The result shows below.</p></td>
<p class=MsoNormal>After pressurized the container, we observed the E.coli by a fluorescence microscope. The result shows below.</p></td>
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       </table>
       </table>
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<table width="100%" border="0" cellspacing="0" cellpadding="0">
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        <tr>
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          <td class="a" width="10%">　</td>
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          <td class="a" width="40%">
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<IMG src="https://static.igem.org/mediawiki/2008/a/af/Tech_acrylic1.JPG
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" align="middle" width="300"></td>
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<td class="a" width="40%"><IMG src="https://static.igem.org/mediawiki/2008/0/01/Tech_acrylic2.JPG
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" align="middle" width="300"></td>
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</table>
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<a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</a>
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<a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</a>
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<a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</a>
   </div>
   </div>
  </body>
  </body>
</html>
</html>

Latest revision as of 11:22, 26 October 2008

Home Construction Acrylic container Development of promoter Genetic toggle switch Parts Submitted to the Registry Result The Team Acknowledgements


Acrylic container

　
　

We create devices for confirming pressure response of lac promoter. This is the first step of creating touch display. This device made of Acrylic glasses and has two holes (show figure). Each hole contains tubes and water. Inside tubes E. coli is cultivated. Pressure can travel to inside tubes. One hole (A) is covered with a plastic tape (show figure). Therefore the hole is pressurized.The other (B) is covered with a block made of an acrylic glass. (show figure) Therefore the hole is not pressurized by water.

　

          

          

　

E.coli type in tubes

 　
　 　

* “Ptet” on pSB6 plasmid (E.coli strain; JM109)

          

          

　

After pressurized the container, we observed the E.coli by a fluorescence microscope. The result shows below.

 　
　
                                

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