Team:Tokyo Tech/Acrylic container

From 2008.igem.org

(Difference between revisions)
 
(12 intermediate revisions not shown)
Line 8: Line 8:
!align="center"|[[Team:Tokyo_Tech/Result|Result]]
!align="center"|[[Team:Tokyo_Tech/Result|Result]]
!align="center"|[[Team:Tokyo_Tech/Team|The Team]]
!align="center"|[[Team:Tokyo_Tech/Team|The Team]]
-
!align="center"|[[Team:Tokyo_Tech/Notebook|Notebook]]
+
!align="center"|[[Team:Tokyo_Tech/Acknowledgements|Acknowledgements]]
|}
|}
Line 38: Line 38:
         </tr>
         </tr>
       </table>
       </table>
-
<br><br>
+
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
 +
 
<table width="100%" border="0" cellspacing="0" cellpadding="0">
<table width="100%" border="0" cellspacing="0" cellpadding="0">
         <tr>  
         <tr>  
Line 50: Line 52:
<table width="100%" border="0" cellspacing="0" cellpadding="0">
<table width="100%" border="0" cellspacing="0" cellpadding="0">
         <tr>  
         <tr>  
-
           <td class="a" width="2%">　<IMG src="https://static.igem.org/mediawiki/2008/1/1b/Tech_aqulyl1.JPG
+
          <td class="a" width="10%">　</td>
 +
           <td class="a" width="2%">　<IMG src="https://static.igem.org/mediawiki/2008/a/a5/Tech_aqulyl1.jpg
" align="middle"></td>
" align="middle"></td>
           <td class="a">
           <td class="a">
-
<p class=MsoNormal>* “Plac” stands for a promoter repressed by lac protein.
+
<p class=MsoNormal>* “Ptet” on pSB6 plasmid (E.coli strain; JM109)</i></p></td>
-
On pSB6 plasmid (E.coli strain; JM109)</i></p></td>
+
-
<td class="a" width="3%">　</td>
+
         </tr>
         </tr>
       </table>
       </table>
-
<br><br>
+
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p><p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
<table width="100%" border="0" cellspacing="0" cellpadding="0">
<table width="100%" border="0" cellspacing="0" cellpadding="0">
         <tr>  
         <tr>  
Line 70: Line 71:
<table width="100%" border="0" cellspacing="0" cellpadding="0">
<table width="100%" border="0" cellspacing="0" cellpadding="0">
         <tr>  
         <tr>  
-
           <td class="a" width="20%">　</td>
+
           <td class="a" width="10%">　</td>
           <td class="a" width="40%">
           <td class="a" width="40%">
<IMG src="https://static.igem.org/mediawiki/2008/a/af/Tech_acrylic1.JPG
<IMG src="https://static.igem.org/mediawiki/2008/a/af/Tech_acrylic1.JPG
-
" align="middle"></td>
+
" align="middle" width="300"></td>
<td class="a" width="40%"><IMG src="https://static.igem.org/mediawiki/2008/0/01/Tech_acrylic2.JPG
<td class="a" width="40%"><IMG src="https://static.igem.org/mediawiki/2008/0/01/Tech_acrylic2.JPG
-
" align="middle"></td>
+
" align="middle" width="300"></td>
</table>
</table>
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
<a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</a>
-
  </div>
+
<a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</a>
-
</body>
+
<a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</a>
-
</html>
+
-
=== Our equipments for pressure experiments===
+
-
<html>
+
-
<body>
+
-
  <div class=Section1 style='layout-grid:18.0pt'>
+
-
 
+
-
<table width="100%" border="0" cellspacing="0" cellpadding="0">
+
-
        <tr>
+
-
          <td class="a" width="2%">　</td>
+
-
          <td class="a">
+
-
<p class=MsoNormal>Dilute this culture medium by 1% by adding fresh medium and suitable antibiotic (ampicilin; 50㎍/ml). Next, seal this culture medium with oxygen-saturated fluorinert (25% volume of medium) in polypropylene tubes with parafilm. (image1) Put this tube into pressure vessel filled with water. (image2) Next cup pressure vessel.
+
-
　      </td>
+
-
      </tr>
+
-
      </table>
+
-
 
+
-
<table width="100%" border="0" cellspacing="0" cellpadding="0">
+
-
        <tr>
+
-
          <td class="a" width="15%">　</td>
+
-
          <td class="a">
+
-
<IMG src="https://static.igem.org/mediawiki/2008/f/f2/Tech_tube_2.JPG" align="middle" width="150">
+
-
</td>
+
-
 
+
-
<td class="a" width="30%">
+
-
 
+
-
</td>
+
-
 
+
-
        </tr>
+
-
      </table>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
<table width="100%" border="0" cellspacing="0" cellpadding="0">
+
-
        <tr>
+
-
          <td class="a" width="2%">　</td>
+
-
          <td class="a">
+
-
(image3)Finally, pressurize pressure vessel by pressure device(image4). and then incubation was started at 37℃ immediately at each pressure vessel(original designed) for 16h at 37℃.After cultivation(image5), the cells were examined by fluorecence microscope.</p></td>
+
-
        </tr>
+
-
      </table>
+
-
 
+
-
 
+
-
<table width="100%" border="0" cellspacing="0" cellpadding="0">
+
-
        <tr>
+
-
          <td class="a" width="15%">　</td>
+
-
          <td class="a">
+
-
<IMG src="https://static.igem.org/mediawiki/2008/d/dc/Tech_equip1.jpg" align="middle">
+
-
</td>
+
-
 
+
-
<td class="a">
+
-
<IMG src="https://static.igem.org/mediawiki/2008/c/c7/Tech_equip2.jpg
+
-
" align="middle">
+
-
</td>
+
-
<td class="a">
+
-
<IMG src="https://static.igem.org/mediawiki/2008/a/a1/Tech_epuip3.jpg
+
-
" align="middle">
+
-
</td>
+
-
        </tr>
+
-
      </table>
+
-
 
+
-
 
+
   </div>
   </div>
  </body>
  </body>
</html>
</html>

Latest revision as of 11:22, 26 October 2008

Home Construction Acrylic container Development of promoter Genetic toggle switch Parts Submitted to the Registry Result The Team Acknowledgements


Acrylic container

　
　

We create devices for confirming pressure response of lac promoter. This is the first step of creating touch display. This device made of Acrylic glasses and has two holes (show figure). Each hole contains tubes and water. Inside tubes E. coli is cultivated. Pressure can travel to inside tubes. One hole (A) is covered with a plastic tape (show figure). Therefore the hole is pressurized.The other (B) is covered with a block made of an acrylic glass. (show figure) Therefore the hole is not pressurized by water.

　

          

          

　

E.coli type in tubes

 　
　 　

* “Ptet” on pSB6 plasmid (E.coli strain; JM109)

          

          

　

After pressurized the container, we observed the E.coli by a fluorescence microscope. The result shows below.

 　
　
                                

Retrieved from "http://2008.igem.org/Team:Tokyo_Tech/Acrylic_container"