Wiki/Team:Warsaw/vectors/pZC320

From 2008.igem.org

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<h2>pMPM-T5-omega vector</h2>
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<h2>pZC320 vector</h2>
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<p class=vector>Description</p><br>
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<p>This vector was a kind gift from the:<br> Department of Bacterial Genetics,<br> Faculty of Biology, <br>University of Warsaw</p><br>
 +
<b>Resistance</b>: ampicillin<br>
 +
<b>Features</b>: One-copy number plasmid, contains LacZ&alpha; fragment under control of T7 promoter<br>
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<b>Usage</b>: This vector was used as a reporter system in blue/white tests for AID and AID fusions activity.
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<img src="https://static.igem.org/mediawiki/2008/3/3d/PMPM-T5-omega.png" width=600>
 
<br><br>
<br><br>
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vector sequence:
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<p class=vector>Vector map</p>
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<br><br>
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<img src="https://static.igem.org/mediawiki/2008/1/1d/PZC320.png" width=600>
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<br><br>
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<p class=vector>Vector sequence</p>
<pre>
<pre>
-
ATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAAC
+
>gi|847861|gb|U26464.1| Cloning vector pZC320, complete sequence
-
CAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAG
+
TCGAGTGAGCGAGGAAGCACCAGGGAACAGCACTTATATATTCTGCTTACACACGATGCCTGAAAAAACT
-
ATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAAC
+
TCCCTTGGGGTTATCCACTTATCCACGGGGATATTTTTATAATTATTTTTTTTATAGTTTTTAGATCTTC
-
GTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCA
+
TTTTTTAGAGCGCCTTGTAGGCCTTTATCCATGCTGGTTCTAGAGAAGGTGTTGTGACAAATTGCCCTTT
-
CTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAA
+
CAGTGTGACAAATCACCCTCAAATGACAGTCCTGTCTGTGACAAATTGCCCTTAACCCTGTGACAAATTG
-
GCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGA
+
CCCTCAGAAGAAGCTGTTTTTTCACAAAGTTATCCCTGCTTATTGACTCTTTTTTATTTAGTGTGACAAT
-
AAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGC
+
CTAAAAACTTGTCACACTTCACATGGATCTGTCATGGCGGAAACAGCGGTTATCAATCACAAGAAACGTA
-
GCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCC
+
AAAATAGCCCGCGAATCGTCCAGTCAAACGACCTCACTGAGGCGGCATATAGTCTCTCCCGGGATCAAAA
-
GCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTG
+
ACGTATGCTGTATCTGTTCGTTGACCAGATCAGAAAATCTGATGGCACCCTACAGGAACATGACGGTATC
-
CGCAACTGTTGGGAAGGGCGCGGGCCATTATTGAAGCATTTATCAGGGTTA
+
TGCGAGATCCATGTTGCTAAATATGCTGAAATATTCGGATTGACCTCTGCGGAAGCCAGTAAGGATATAC
-
TTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAAA
+
GGCAGGCATTGAAGAGTTTCGCGGGGAAGGAAGTGGTTTTTTATCGCCCTGAAGAGGATGCCGGCGATGA
-
GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGACTGTCTGCTTAATTTG
+
AAAAGGCTATGAATCTTTTCCTTGGTTTATCAAACGTGCGCACAGTCCATCCAGAGGGCTTTACAGTGTA
-
ATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTG
+
CATATCAACCCATATCTCATTCCCTTCTTTATCGGGTTACAGAACCGGTTTACGCAGTTTCGGCTTAGTG
-
CTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGC
+
AAACAAAAGAAATCACCAATCCGTATGCCATGCGTTTATACGAATCCCTGTGTCAGTATCGTAAGCCGGA
-
GTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGC
+
TGGCTCAGGCATCGTCTCTCTGAAAATCGACTGGATCATAGAGCGTTACCAGCTGCCTCAAAGTTACCAG
-
CTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGAAGACC
+
CGTATGCCTGACTTCCGCCGCCGCTTCCTGCAGGTCTGTGTTAATGAGATCAACAGCAGAACTCCAATGC
-
CACACTACCATCGGGCCTACGGGCTTTCACTTCTAGTTGGGCATGGGGTCA
+
GCCTCTCATACATTGAGAAAAAGAAAGGCCGCCAGACGACTCATATCGTATTTTCCTTCCGCGATATCAC
-
GGTGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACGCT
+
TTCCATGACGACAGGATAGTCTGAGGGTTATCTGTCACAGATTTGAGGGTGGTTCGTCACATTTGTTCTG
-
TATGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCC
+
ACCTACTGAGGGTAATTTGTCACAGTTTTGCTGTTTCCTTCAGCCTGCATGGATTTTCTCATACTTTTTG
-
AAAACAGCCAAGCTGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGAT
+
AACTGTAATTTTTAAGGAAGCCAAATTTGAGGGCAGTTTGTCACAGTTGATTTCCTTCTCTTTCCCTTCG
-
ATCGCATGCCTGCAGCCCGGGAGATCTACTAGTTCTAGagctttatgcttg
+
TCATGTGACCTGATATCGGGGGTTAGTTCGTCATCATTGATGAGGGTTGATTATCACAGTTTATTACTCT
-
taaaccgttttgtgaaaaaatttttaaaataaaaaaggggacctctagggt
+
GAATTGGCTATCCGCGTGTGTACCTCTACCTGGAGTTTTTCCCACGGTGGATATTTCTTCTTGCGCTGAG
-
ccccaattaattagtaatataatctattaaaggtcattcaaaaggtcatcc
+
CGTAAGAGCTATCTGACAGAACAGTTCTTCTTTGCTTCCTCGCCAGTTCGCTCGCTATGCTCGGTTACAC
-
accggatcagcttagtaaagccctcgctagattttaatgcggatgttgcga
+
GGCTGCGGCGAGCGCTAGTGATAATAAGTGACTGAGGTATGTGCTCTTCTTATCTCCTTTTGTAGTGTTG
-
ttacttcgccaactattgcgataacaagaaaaagccagcctttcatgatat
+
CTCTTATTTTAAACAACTTTGCGGTTTTTTGATGACTTTGCGATTTTGTTGTTGCTTTGCAGTAAATTGC
-
atctcccaatttgtgtagggcttattatgcacgcttaaaaataataaaagc
+
AAGATTTAATAAAAAAACGCAAAGCAATGATTAAAGGATGTTCAGAATGAAACTCATGGAAACACTTAAC
-
agacttgacctgatagtttggctgtgagcaattatgtgcttagtgcatcta
+
CAGTGCATAAACGCTGGTCATGAAATGACGAAGGCTATCGCCATTGCACAGTTTAATGATGACAGCCCGG
-
acgcttgagttaagccgcgccgcgaagcggcgtcggcttgaacgaattgtt
+
AAGCGAGGAAAATAACCCGGCGCTGGAGAATAGGTGAAGCAGCGGATTTAGTTGGGGTTTCTTCTCAGGC
-
agacattatttgccgactaccttggtgatctcgcctttcacgtagtggaca
+
TATCAGAGATGCCGAGAAAGCAGGGCGACTACCGCACCCGGATATGGAAATTCGAGGACGGGTTGAGCAA
-
aattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtcca
+
CGTGTTGGTTATACAATTGAACAAATTAATCATATGCGTGATGTGTTTGGTACGCGATTGCGACGTGCTG
-
agataagcctgtctagcttcaagtatgacgggctgatactgggccggcagg
+
AAGACGTATTTCCACCGGTGATCGGGGTTGCTGCCCATAAAGGTGGCGTTTACAAAACCTCAGTTTCTGT
-
cgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggtt
+
TCATCTTGCTCAGGATCTGGCTCTGAAGGGGCTACGTGTTTTGCTCGTGGAAGGTAACGACCCCCAGGGA
-
actgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcg
+
ACAGCCTCAATGTATCACGGATGGGTACCAGATCTTCATATTCATGCAGAAGACACTCTCCTGCCTTTCT
-
ccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcc
+
ATCTTGGGGAAAAGGACGATGTCACTTATGCAATAAAGCCCACTTGCTGGCCGGGGCTTGACATTATTCC
-
tcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctgga
+
TTCCTGTCTGGCTCTGCACCGTATTGAAACTGAGTTAATGGGCAAATTTGATGAAGGTAAACTGCCCACC
-
cctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccaga
+
GATCCACACCTGATGCTCCGACTGGCCATTGAAACTGTTGCTCATGACTATGATGTCATAGTTATTGACA
-
tcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgc
+
GCGCGCCTAACCTGGGTATCGGCACGATTAATGTCGTATGTGCTGCTGATGTGCTGATTGTTCCCACGCC
-
tgccattctccaaattgcagttcgcgcttagctggataacgccacggaatg
+
TGCTGAGTTGTTTGACTACACCTCCGCACTGCAGTTTTTCGATATGCTTCGTGATCTGCTCAAGAACGTT
-
atgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctc
+
GATCTTAAAGGGTTCGAGCCTGATGTACGTATTTTGCTTACCAAATACAGCAATAGTAATGGCTCTCAGT
-
tctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgc
+
CCCCGTGGATGGAGGAGCAAATTCGGGATGCCTGGGGAAGCATGGTTCTAAAAAATGTTGTACGTGAAAC
-
gttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactg
+
GGATGAAGTTGGTAAAGGTCAGATCCGGATGAGAACTGTTTTTGAACAGGCCATTGATCAACGCTCTTCA
-
tgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagc
+
ACTGGTGCCTGGAGAAATGCTCTTTCTATTTGGGAACCTGTCTGCAATGAAATTTTCGATCGTCTGATTA
-
aacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttga
+
AACCACGCTGGGAGATTAGATAATGAAGCGTGCGCCTGTTATTCCAAAACATACGCTCAATACTCAACCG
-
gtcgatacttcggcgatcaccgcttccctcatgatgtttaactttgtttta
+
GTTGAAGATACTTCGTTATCGACACCAGCTGCCCCGATGGTGGATTCGTTAATTGCGCGCGTAGGAGTAA
-
gggcgactgccctgctgcgtaacatcgttgctgctccataacatcaaacat
+
TGGCTCGCGGTAATGCCATTACTTTGCCTGTATGTGGTCGGGATGTGAAGTTTACTCTTGAAGTGCTCCG
-
cgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgt
+
GGGTGATAGTGTTGAGAAGACCTCTCGGGTATGGTCAGGTAATGAACGTGACCAGGAGCTGCTTACTGAG
-
accccaaaaaaacagtcataacaagccatgaaaaccgccactgcgccgtta
+
GACGCACTGGATGATCTCATCCCTTCTTTTCTACTGACTGGTCAACAGACACCGGCGTTCGGTCGAAGAG
-
ccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgc
+
TATCTGGTGTCATAGAAATTGCCGATGGGAGTCGCCGTCGTAAAGCTGCTGCACTTACCGAAAGTGATTA
-
tacttgcattacagcttacgaaccgaacaggcttatgtccactgggttcgt
+
TCGTGTTCTGGTTGGCGAGCTGGATGATGAGCAGATGGCTGCATTATCCAGATTGGGTAACGATTATCGC
-
gccttcatccgtttccacggtgtgcgtcacccggcaaccttgggcagcagc
+
CCAACAAGTGCTTATGAACGTGGTCAGCGTTATGCAAGCCGATTGCAGAATGAATTTGCTGGAAATATTT
-
gaagtcgaggcatttctgtcctggctggcgaacgagcgcaaggtttcggtc
+
CTGCGCTGGCTGATGCGGAAAATATTTCACGTAAGATTATTACCCGCTGTATCAACACCGCCAAATTGCC
-
tccacgcatcgtcaggcattggcggccttgctgttcttctacggcaaggtg
+
TAAATCAGTTGTTGCTCTTTTTTCTCACCCCGGTGAACTATCTGCCCGGTCAGGTGATGCACTTCAAAAA
-
ctgtgcacggatctgccctggcttcaggagatcggaagacctcggccgtcg
+
GCCTTTACAGATAAAGAGGAATTACTTAAGCAGCAGGCATCTAACCTTCATGAGCAGAAAAAAGCTGGGG
-
cggcgcttgccggtggtgctgaccccggatgaagtggttcgcatcctcggt
+
TGATATTTGAAGCTGAAGAAGTTATCACTCTTTTAACTTCTGTGCTTAAAACGTCATCTGCATCAAGAAC
-
tttctggaaggcgagcatcgtttgttcgcccagcttctgtatggaacgggc
+
TAGTTTAAGCTCACGACATCAGTTTGCTCCTGGAGCGACAGTATTGTATAAGGGCGATAAAATGGTGCTT
-
atgcggatcagtgagggtttgcaactgcgggtcaaggatctggatttcgat
+
AACCTGGACAGGTCTCGTGTTCCAACTGAGTGTATAGAGAAAATTGAGGCCATTCTTAAGGAACTTGAAA
-
cacggcacgatcatcgtgcgggagggcaagggctccaaggatcgggccttg
+
AGCCAGCACCCTGATGCGACCACGTTTTAGTCTACGTTTATCTGTCTTTACTTAATGTCCTTTGTTACAG
-
atgttacccgagagcttggcacccagcctgcgcgagcaggggaattgatcc
+
GCCAGAAAGCATAACTGGCCTGAATATTCTCTCTGGGCCCACTGTTCCACTTGTATCGTCGGTCTGATAA
-
ggtggatgaccttttgaatgacctttaatagattatattactaattaattg
+
TCAGACTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGT
-
gggaccctagaggtccccttttttattttaaaaattttttcacaaaacggt
+
ATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATAATCAGACTGGGA
-
ttacaagcataaagctCTAGAGCCATGGTGAATTCCTCCTTTCACTCCATC
+
CCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCATGGTCCCACTCGTATCGTCGGTCT
-
CAAAAAAACGGGTATGGAGAAACAGTAGAGAGTTGCGATAAAAAGCGTCAG
+
GATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGAACCACGGTCCCA
-
GTAGGATCCGCTAATCTTATGGATAAAAATGCTATGGCATAGCAAAGTGTG
+
CTCGTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTC
-
ACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTT
+
TGGGACCACGATCCCACTCGTGTTGTCGGTCTGATTATCGGTCTGGGACCACGGTCCCACTTGTATTGTC
-
TTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTT
+
GATCAGACTATCAGCGTGAGACTACGATTCCATCAATGCCTGTCAAGGGCAAGTATTGACATGTCGTCGT
-
TTAATAAGCGGGGTTACCGGTTGGGTTAGCGAGAAGAGCCAGTAAAAGACG
+
AACCTGTAGAACGGAGTAACCTCGGTGTGCGGTTGTATGCCTGCTGTGGATTGCTGCTGTGTCCTGCTTA
-
CAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAAT
+
TCCACAACATTTTGCGCACGGTTATGTGGACAAAATACCTGGTTACCCAGGCCGTGCCGGCACGTTAACC
-
GGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCG
+
GGGCTGCATCCGATGCAAGTGTGTCGCTGTCGACGGCCTCCTCACCCGGTCACGTTTCGTCGTTTCTCCT
-
GGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCC
+
CCACGCGCTCGGCTTCGGGGCCGCACCTGCATTCGTATGCGGTCGCCCGGTTACAGGTGCGGCACGGCCT
-
AACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTAT
+
GATGGAGGCCGCATGTGAGAGGAGAATTCCCGGGGATCAGCTTTATGCTTGTAAACCGTTTTGTGAAAAA
-
ATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGA
+
ATTTTTAAAATAAAAAAGGGGACCTCTAGGGTCCCCAATTAATTAGTAATATAATCTATTAAAGGTCATT
-
GAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATT
+
CAAAAGGTCATCCACCGGATCAGCTTAGTAAAGCCCTCGCTAGATTTTAATGCGGATGTTGCGATTACTT
-
CATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTT
+
CGCCAACTATTGCGATAACAAGAAAAAGCCAGCCTTTCATGATATATCTCCCAATTTGTGTAGGGCTTAT
-
TACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATA
+
TATGCACGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCTGTGAGCAATTATGTGCTTAGT
-
TTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTC
+
GCATCTAACGCTTGAGTTAAGCCGCGCCGCGAAGCGGCGCATGAGATTATCAAAAAGGATCTTCACCTAG
-
AGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTAT
+
ATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT
-
TCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCATG
+
ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACT
-
GAAGCGATTAACGAGTCGCTCCATCCGCCGATGGATAATCGGGTACGCGAG
+
CCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGA
-
GCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCC
+
GACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTG
-
AGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTC
+
GTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCC
-
CGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATC
+
AGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG
-
AGCCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTC
+
GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGG
-
GGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAA
+
TTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGC
-
AAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAA
+
AGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACC
-
GTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATT
+
AAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCG
-
GACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATT
+
CGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT
-
TGACAGGCCACATTATGCAAGCATTGCGGTAATGAATCGGCCAACGCGCGG
+
CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACT
-
GGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACT
+
TTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACAC
-
CGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGG
+
GGAAATGTTGAATACTCATACTCTTCCTTTTTCAATCGCCAACTACCTCTGATAGTTGAGTCGATACTTC
-
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGG
+
GGCGATCACCGCTTCCCTCATGATGTTTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTG
-
CATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAAC
+
CTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACT
-
GAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCA
+
GTACCCCAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGT
-
TACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATC
+
CAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGCTTACGAACCGAACAGGCTTAT
-
ATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTAC
+
GTCCACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAG
-
CCCCATGAACAGAAATTCCCCCTTACACGGAGGCATCAAGTGACCAAACAG
+
TCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGC
-
GAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACG
+
GGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCT
-
CTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGT
+
CGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAG
-
GAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGT
+
GCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGATTAACGCTTACAATTTCCATTCGCCATTC
-
TTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTC
+
AGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGG
-
ACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCG
+
GATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGC
-
TCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGC
+
CAGTGAGCGCGCCGGATCCACGTGATCGATGCATACGCGTTCGAAAGCTTCGCGACGTACGACGTCCGCG
-
GATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTAC
+
GAGCTCTCGAGCATGCTAGCGGCCGCCCTATAGTGAGTCGTATTACGCGCGCTTGGCGTAATCATGGTCA
-
TGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAG
+
TAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGT
-
AAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCG
+
GTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCA
-
CTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAAT
+
GTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGG
-
ACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAA
+
ATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTT
-
AGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTT
+
ACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGGGGAATTGATCCGGTGGATGACCTTTTGAATGACCT
-
CCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA
+
TTAATAGATTATATTACTAATTAATTGGGGACCCTAGAGGTCCCCTTTTTTATTTTAAAAATTTTTTCAC
-
GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGG
+
AAAACGGTTTACAAGCATAAAGCTGATCCCCGGGAATT
-
AAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCT
+
-
GTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTG
+
-
TAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCA
+
-
CGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCT
+
-
TGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGG
+
-
TAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA
+
-
GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGC
+
-
TCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG
+
-
CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGAT
+
-
TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG
+
-
GTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGCC
+
-
GAGATGCGCCGCGTGCGGCTGCTGGAGATGGCGGACGCGATGGATATGTTC
+
-
TGCCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCT
+
-
CCAATTCTTGGAGTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTC
+
-
AGGTCGAGGTGGCCCGGCTCCATGCACCGCGACGCAACGCGGGGAGGCAGA
+
-
CAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCG
+
-
CCGAGGCGGCATAAATCGCCGTGACGATCAGCGGTCCAGTGATCGAAGTTA
+
-
GGCTGGTAAGAGCCGCGAGCGATCCTTGAAGCTGTCCCTGATGGTCGTCAT
+
-
CTACCTGCCTGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGG
+
-
AAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGCGAACG
+
-
CCAGCAAGACGTAGCCCAGCGCGTCGGCCGCCATGCCGGCGATAATGGCCT
+
-
GCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGA
+
-
GGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGC
+
-
TCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCT
+
-
GTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAG
+
-
TCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGG
+
-
GCATCGGTCGGCGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCA
+
-
GTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGTA
+
-
AGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCA
+
-
CGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCAT
+
-
CGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGA
+
-
TGCCGGCCACGATGCGTCCGGCGTAGAGAATCCACAGGACGGGTGTGGTCG
+
-
CCATGATCGCGTAGTCGATAGTGGCTCCAAGTAGCGAAGCGAGCAGGACTG
+
-
GGCGGCGGCCAAAGCGGTCGGACAGTGCTCCGAGAACGGGTGCGCATAGAA
+
-
ATTGCATCAACGCATATAGCGCTAGCAGCACGCCATAGTGACTGGCGATGC
+
-
TGTCGGAATGGACGATATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCA
+
-
AGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGATGACGATGAGCG
+
-
CATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTG
+
-
ATAAACTACCGCATTAAAGCTAATCGATGATAAGCTGTCAAACATGAGAAT
+
-
T
+
</pre>
</pre>

Latest revision as of 11:57, 26 October 2008

Gallery Bricks Notebook Team Project Home


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Vectors view

 

pZC320 vector

Description


This vector was a kind gift from the:
Department of Bacterial Genetics,
Faculty of Biology,
University of Warsaw


Resistance: ampicillin
Features: One-copy number plasmid, contains LacZα fragment under control of T7 promoter
Usage: This vector was used as a reporter system in blue/white tests for AID and AID fusions activity.

Vector map





Vector sequence

>gi|847861|gb|U26464.1| Cloning vector pZC320, complete sequence
TCGAGTGAGCGAGGAAGCACCAGGGAACAGCACTTATATATTCTGCTTACACACGATGCCTGAAAAAACT
TCCCTTGGGGTTATCCACTTATCCACGGGGATATTTTTATAATTATTTTTTTTATAGTTTTTAGATCTTC
TTTTTTAGAGCGCCTTGTAGGCCTTTATCCATGCTGGTTCTAGAGAAGGTGTTGTGACAAATTGCCCTTT
CAGTGTGACAAATCACCCTCAAATGACAGTCCTGTCTGTGACAAATTGCCCTTAACCCTGTGACAAATTG
CCCTCAGAAGAAGCTGTTTTTTCACAAAGTTATCCCTGCTTATTGACTCTTTTTTATTTAGTGTGACAAT
CTAAAAACTTGTCACACTTCACATGGATCTGTCATGGCGGAAACAGCGGTTATCAATCACAAGAAACGTA
AAAATAGCCCGCGAATCGTCCAGTCAAACGACCTCACTGAGGCGGCATATAGTCTCTCCCGGGATCAAAA
ACGTATGCTGTATCTGTTCGTTGACCAGATCAGAAAATCTGATGGCACCCTACAGGAACATGACGGTATC
TGCGAGATCCATGTTGCTAAATATGCTGAAATATTCGGATTGACCTCTGCGGAAGCCAGTAAGGATATAC
GGCAGGCATTGAAGAGTTTCGCGGGGAAGGAAGTGGTTTTTTATCGCCCTGAAGAGGATGCCGGCGATGA
AAAAGGCTATGAATCTTTTCCTTGGTTTATCAAACGTGCGCACAGTCCATCCAGAGGGCTTTACAGTGTA
CATATCAACCCATATCTCATTCCCTTCTTTATCGGGTTACAGAACCGGTTTACGCAGTTTCGGCTTAGTG
AAACAAAAGAAATCACCAATCCGTATGCCATGCGTTTATACGAATCCCTGTGTCAGTATCGTAAGCCGGA
TGGCTCAGGCATCGTCTCTCTGAAAATCGACTGGATCATAGAGCGTTACCAGCTGCCTCAAAGTTACCAG
CGTATGCCTGACTTCCGCCGCCGCTTCCTGCAGGTCTGTGTTAATGAGATCAACAGCAGAACTCCAATGC
GCCTCTCATACATTGAGAAAAAGAAAGGCCGCCAGACGACTCATATCGTATTTTCCTTCCGCGATATCAC
TTCCATGACGACAGGATAGTCTGAGGGTTATCTGTCACAGATTTGAGGGTGGTTCGTCACATTTGTTCTG
ACCTACTGAGGGTAATTTGTCACAGTTTTGCTGTTTCCTTCAGCCTGCATGGATTTTCTCATACTTTTTG
AACTGTAATTTTTAAGGAAGCCAAATTTGAGGGCAGTTTGTCACAGTTGATTTCCTTCTCTTTCCCTTCG
TCATGTGACCTGATATCGGGGGTTAGTTCGTCATCATTGATGAGGGTTGATTATCACAGTTTATTACTCT
GAATTGGCTATCCGCGTGTGTACCTCTACCTGGAGTTTTTCCCACGGTGGATATTTCTTCTTGCGCTGAG
CGTAAGAGCTATCTGACAGAACAGTTCTTCTTTGCTTCCTCGCCAGTTCGCTCGCTATGCTCGGTTACAC
GGCTGCGGCGAGCGCTAGTGATAATAAGTGACTGAGGTATGTGCTCTTCTTATCTCCTTTTGTAGTGTTG
CTCTTATTTTAAACAACTTTGCGGTTTTTTGATGACTTTGCGATTTTGTTGTTGCTTTGCAGTAAATTGC
AAGATTTAATAAAAAAACGCAAAGCAATGATTAAAGGATGTTCAGAATGAAACTCATGGAAACACTTAAC
CAGTGCATAAACGCTGGTCATGAAATGACGAAGGCTATCGCCATTGCACAGTTTAATGATGACAGCCCGG
AAGCGAGGAAAATAACCCGGCGCTGGAGAATAGGTGAAGCAGCGGATTTAGTTGGGGTTTCTTCTCAGGC
TATCAGAGATGCCGAGAAAGCAGGGCGACTACCGCACCCGGATATGGAAATTCGAGGACGGGTTGAGCAA
CGTGTTGGTTATACAATTGAACAAATTAATCATATGCGTGATGTGTTTGGTACGCGATTGCGACGTGCTG
AAGACGTATTTCCACCGGTGATCGGGGTTGCTGCCCATAAAGGTGGCGTTTACAAAACCTCAGTTTCTGT
TCATCTTGCTCAGGATCTGGCTCTGAAGGGGCTACGTGTTTTGCTCGTGGAAGGTAACGACCCCCAGGGA
ACAGCCTCAATGTATCACGGATGGGTACCAGATCTTCATATTCATGCAGAAGACACTCTCCTGCCTTTCT
ATCTTGGGGAAAAGGACGATGTCACTTATGCAATAAAGCCCACTTGCTGGCCGGGGCTTGACATTATTCC
TTCCTGTCTGGCTCTGCACCGTATTGAAACTGAGTTAATGGGCAAATTTGATGAAGGTAAACTGCCCACC
GATCCACACCTGATGCTCCGACTGGCCATTGAAACTGTTGCTCATGACTATGATGTCATAGTTATTGACA
GCGCGCCTAACCTGGGTATCGGCACGATTAATGTCGTATGTGCTGCTGATGTGCTGATTGTTCCCACGCC
TGCTGAGTTGTTTGACTACACCTCCGCACTGCAGTTTTTCGATATGCTTCGTGATCTGCTCAAGAACGTT
GATCTTAAAGGGTTCGAGCCTGATGTACGTATTTTGCTTACCAAATACAGCAATAGTAATGGCTCTCAGT
CCCCGTGGATGGAGGAGCAAATTCGGGATGCCTGGGGAAGCATGGTTCTAAAAAATGTTGTACGTGAAAC
GGATGAAGTTGGTAAAGGTCAGATCCGGATGAGAACTGTTTTTGAACAGGCCATTGATCAACGCTCTTCA
ACTGGTGCCTGGAGAAATGCTCTTTCTATTTGGGAACCTGTCTGCAATGAAATTTTCGATCGTCTGATTA
AACCACGCTGGGAGATTAGATAATGAAGCGTGCGCCTGTTATTCCAAAACATACGCTCAATACTCAACCG
GTTGAAGATACTTCGTTATCGACACCAGCTGCCCCGATGGTGGATTCGTTAATTGCGCGCGTAGGAGTAA
TGGCTCGCGGTAATGCCATTACTTTGCCTGTATGTGGTCGGGATGTGAAGTTTACTCTTGAAGTGCTCCG
GGGTGATAGTGTTGAGAAGACCTCTCGGGTATGGTCAGGTAATGAACGTGACCAGGAGCTGCTTACTGAG
GACGCACTGGATGATCTCATCCCTTCTTTTCTACTGACTGGTCAACAGACACCGGCGTTCGGTCGAAGAG
TATCTGGTGTCATAGAAATTGCCGATGGGAGTCGCCGTCGTAAAGCTGCTGCACTTACCGAAAGTGATTA
TCGTGTTCTGGTTGGCGAGCTGGATGATGAGCAGATGGCTGCATTATCCAGATTGGGTAACGATTATCGC
CCAACAAGTGCTTATGAACGTGGTCAGCGTTATGCAAGCCGATTGCAGAATGAATTTGCTGGAAATATTT
CTGCGCTGGCTGATGCGGAAAATATTTCACGTAAGATTATTACCCGCTGTATCAACACCGCCAAATTGCC
TAAATCAGTTGTTGCTCTTTTTTCTCACCCCGGTGAACTATCTGCCCGGTCAGGTGATGCACTTCAAAAA
GCCTTTACAGATAAAGAGGAATTACTTAAGCAGCAGGCATCTAACCTTCATGAGCAGAAAAAAGCTGGGG
TGATATTTGAAGCTGAAGAAGTTATCACTCTTTTAACTTCTGTGCTTAAAACGTCATCTGCATCAAGAAC
TAGTTTAAGCTCACGACATCAGTTTGCTCCTGGAGCGACAGTATTGTATAAGGGCGATAAAATGGTGCTT
AACCTGGACAGGTCTCGTGTTCCAACTGAGTGTATAGAGAAAATTGAGGCCATTCTTAAGGAACTTGAAA
AGCCAGCACCCTGATGCGACCACGTTTTAGTCTACGTTTATCTGTCTTTACTTAATGTCCTTTGTTACAG
GCCAGAAAGCATAACTGGCCTGAATATTCTCTCTGGGCCCACTGTTCCACTTGTATCGTCGGTCTGATAA
TCAGACTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGT
ATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATAATCAGACTGGGA
CCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCATGGTCCCACTCGTATCGTCGGTCT
GATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGAACCACGGTCCCA
CTCGTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTC
TGGGACCACGATCCCACTCGTGTTGTCGGTCTGATTATCGGTCTGGGACCACGGTCCCACTTGTATTGTC
GATCAGACTATCAGCGTGAGACTACGATTCCATCAATGCCTGTCAAGGGCAAGTATTGACATGTCGTCGT
AACCTGTAGAACGGAGTAACCTCGGTGTGCGGTTGTATGCCTGCTGTGGATTGCTGCTGTGTCCTGCTTA
TCCACAACATTTTGCGCACGGTTATGTGGACAAAATACCTGGTTACCCAGGCCGTGCCGGCACGTTAACC
GGGCTGCATCCGATGCAAGTGTGTCGCTGTCGACGGCCTCCTCACCCGGTCACGTTTCGTCGTTTCTCCT
CCACGCGCTCGGCTTCGGGGCCGCACCTGCATTCGTATGCGGTCGCCCGGTTACAGGTGCGGCACGGCCT
GATGGAGGCCGCATGTGAGAGGAGAATTCCCGGGGATCAGCTTTATGCTTGTAAACCGTTTTGTGAAAAA
ATTTTTAAAATAAAAAAGGGGACCTCTAGGGTCCCCAATTAATTAGTAATATAATCTATTAAAGGTCATT
CAAAAGGTCATCCACCGGATCAGCTTAGTAAAGCCCTCGCTAGATTTTAATGCGGATGTTGCGATTACTT
CGCCAACTATTGCGATAACAAGAAAAAGCCAGCCTTTCATGATATATCTCCCAATTTGTGTAGGGCTTAT
TATGCACGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCTGTGAGCAATTATGTGCTTAGT
GCATCTAACGCTTGAGTTAAGCCGCGCCGCGAAGCGGCGCATGAGATTATCAAAAAGGATCTTCACCTAG
ATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT
ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACT
CCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGA
GACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTG
GTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCC
AGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG
GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGG
TTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGC
AGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACC
AAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCG
CGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT
CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACT
TTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACAC
GGAAATGTTGAATACTCATACTCTTCCTTTTTCAATCGCCAACTACCTCTGATAGTTGAGTCGATACTTC
GGCGATCACCGCTTCCCTCATGATGTTTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTG
CTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACT
GTACCCCAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGT
CAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGCTTACGAACCGAACAGGCTTAT
GTCCACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAG
TCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGC
GGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCT
CGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAG
GCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGATTAACGCTTACAATTTCCATTCGCCATTC
AGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGG
GATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGC
CAGTGAGCGCGCCGGATCCACGTGATCGATGCATACGCGTTCGAAAGCTTCGCGACGTACGACGTCCGCG
GAGCTCTCGAGCATGCTAGCGGCCGCCCTATAGTGAGTCGTATTACGCGCGCTTGGCGTAATCATGGTCA
TAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGT
GTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCA
GTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGG
ATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTT
ACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGGGGAATTGATCCGGTGGATGACCTTTTGAATGACCT
TTAATAGATTATATTACTAATTAATTGGGGACCCTAGAGGTCCCCTTTTTTATTTTAAAAATTTTTTCAC
AAAACGGTTTACAAGCATAAAGCTGATCCCCGGGAATT