Team:Chiba/Calendar-Home/16 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Team:Communication)
Line 6: Line 6:
===Team:Communication===
===Team:Communication===
-
(15/9)->
+
(15/9)->Transformants->[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
-
[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
+
-
 
+
-
 
+
*reaction temparaturewas 37°C and 30°C.
*reaction temparaturewas 37°C and 30°C.

Revision as of 12:31, 26 October 2008

>Home | Notebook

15 September 2008 <|> 17 September 2008

Laboratory work

Team:Communication

(15/9)->Transformants->time-delay test

  • reaction temparaturewas 37°C and 30°C.

Team:Output


Digestion test

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(x2)
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(x5)
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(x2)
  • [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
  • [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
  • [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](RBS+cinR)
Sample No. each
DNA(μL) 3
EcoRⅠ(μL) 0.083
PstⅠ(μL) 0.083
BSA(x10)(μL) 1
Buffer3(x10)(μL) 1
dH2O(μL) 5
TOTAL(μL) 10

-->37℃ 1hour

Gel check

Sample No. each
DNA(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12

Transformation

  • [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](LuxI+RBS+LuxR)->no colony
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](LuxI+LuxR+pLux+GFP)
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

PCR

  • Venus YFP(ptet+LuxR+YFP)
Sample No. YFP
DNA(μL) 1
Venus Fwd(μL) 5
Rev(VR)(μL) 5
Thermo pol Buffer(μL) 10
dNTP mix(μL) 10
Vent(μL) 1
dH2O(μL) 68
TOTAL(μL) 100
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/16_September_2008"