Team:Warsaw/Calendar-Main/21 May 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

Inoculation of separate transformant colonies for transcriptional fusion (liquid LB + tetracycline).
Inoculation of separate transformant colonies for translational fusion (liquid LB + tetracycline).

Rifampicin test #1

Michał L., Ewa, Marcin

Strain	Inducer / repressor	Number of colonies on rifampicin
Top10	none	12
Top10	0.5 mM IPTG	17
Top10 pMPM-T5-AID	none	13
Top10 pMPM-T5-AID	0.1% Glucose	18
Top10 pMPM-T5-AID	0.1% Arabinose	54
Top10 pTrc99a-AID	none	14
Top10 pTrc99a-AID	1% Glucose	17
Top10 pTrc99a-AID	0.5 mM IPTG	53

Conclusion: AID seems to work!

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+<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a></h3>
+<h4>Michał K.</h4>
+<p><ol>
+<li> Inoculation of separate transformant colonies for <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>transcriptional fusion</a> (liquid LB + tetracycline).</li>
+<li> Inoculation of separate transformant colonies for <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>translational fusion</a> (liquid LB + tetracycline). </li>
+</ol></p>
-<p>Michał K: <br>
+<h3> Rifampicin test #1</h3>
-. PCR - transcription fusion: AID + T7 RNA-polymerase - optimalization (temperature gradient 60&deg;C - 80&deg;C).</p>
+<h4>Michał L., Ewa, Marcin</h4>
-<p>Primers: </p>
-<p><html>
-<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and
-<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></html></p>
-Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br>
-Annealing time: 4 minutes <br>
-cycles <br>
-. Gel electrophoresis. <br>
+<table id="result">
+<tr><th>Strain</th><th>Inducer / repressor</th><th>Number of colonies on rifampicin</th></tr>
+<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a></th><td>none</td><td>12</td></tr>
+<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a></th><td>0.5 mM IPTG</td><td>17</td></tr>
+<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>none</td><td>13</td></tr>
+<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>0.1% Glucose</td><td>18</td></tr>
+<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>0.1% Arabinose</td><td>54</td></tr>
+<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a></th><td>none</td><td>14</td></tr>
+<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a></th><td>1% Glucose</td><td>17</td></tr>
+<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a></th><td>0.5 mM IPTG</td><td>53</td></tr>
+</table>
+<br/>
+<p>Conclusion: AID seems to work!</p>
+</html>
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