Team:Warsaw/Calendar-Main/23 May 2008

From 2008.igem.org

(Difference between revisions)
 
(29 intermediate revisions not shown)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<html>
-
1. Digest of pMPM-T5 with NcoI and XbaI
+
<h3>Rifampicin test #2</h3>
 +
<h4>Piotr</h4>
-
2. Digest of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI
+
<p> Plating cultures from the previous day on rifampicin.</p>
-
3. Clean-up of digested products from p.1 and p.2
 
-
4. Ligation of digested products from p.1 and p.2
+
<h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a></h3>
 +
<h4>Michał K.</h4>
-
5. Transformation of E.coli TOP10 with ligation product
+
<p> Further inoculation of separate transformant colonies for transcriptional and translational fusions on liquid LB + tetracycline (as there were no positive colonies in previous assay).</p>
-
6. Digest of pMPM-T5+AID with HindIII and SalI
 
-
7. Digest of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI
+
</html>
-
8. Clean-up of digested products from p.6 and p.7
 
-
9. Ligation of digested products from p.6 and p.7
 
-
 
-
10. Transformation of E.coli TOP10 with ligation product
 
-
 
-
11. Transformants plating on LB + tetracycline
 
-
 
-
 
-
http://openwetware.org/images/thumb/6/61/UW_A%2BT.jpg/350px-UW_A%2BT.jpg http://openwetware.org/images/thumb/c/c7/UW_AT.jpg/350px-UW_AT.jpg
 
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Latest revision as of 14:08, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Rifampicin test #2

Piotr

Plating cultures from the previous day on rifampicin.

Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

Further inoculation of separate transformant colonies for transcriptional and translational fusions on liquid LB + tetracycline (as there were no positive colonies in previous assay).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/23_May_2008"