Team:Warsaw/Calendar-Main/23 May 2008

From 2008.igem.org

(Difference between revisions)
 
(21 intermediate revisions not shown)
Line 4: Line 4:
<html>
<html>
-
<p>Michał K.:</p>
 
-
<p>1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digestation</a> of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI (2x Tango buffer).</p>
+
<h3>Rifampicin test #2</h3>
 +
<h4>Piotr</h4>
-
<p>2. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digestation</a> of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI</p>
+
<p> Plating cultures from the previous day on rifampicin.</p>
-
<p>3. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of digested products from p.1 and p.2</p>
 
-
<p>4. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digestation</a> of pMPM-T5+AID with HindIII and SalI (2x Tango buffer).</p>
+
<h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a></h3>
 +
<h4>Michał K.</h4>
-
<p>5. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digestation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with NcoI and XbaI</p>
+
<p> Further inoculation of separate transformant colonies for transcriptional and translational fusions on liquid LB + tetracycline (as there were no positive colonies in previous assay).</p>
-
<p>6. Gel-electrophresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands.</p>
 
-
<p>7. Ligation of purified products from p.1 and p.4</p>
+
</html>
-
 
+
-
<p>8. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of E.coli TOP10 with ligation product</p>
+
-
 
+
-
<p>9. Ligation of digested products from p.4 and p.5</p>
+
-
 
+
-
<p>10. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of E.coli TOP10 with ligation product</p>
+
-
 
+
-
<p>11. Transformants plating on LB + tetracycline</p>
+
-
 
+
-
</html>
+
-
 
+
-
http://openwetware.org/images/thumb/6/61/UW_A%2BT.jpg/350px-UW_A%2BT.jpg http://openwetware.org/images/thumb/c/c7/UW_AT.jpg/350px-UW_AT.jpg
+

Latest revision as of 14:08, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Rifampicin test #2

Piotr

Plating cultures from the previous day on rifampicin.

Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

Further inoculation of separate transformant colonies for transcriptional and translational fusions on liquid LB + tetracycline (as there were no positive colonies in previous assay).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/23_May_2008"