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Team:Imperial College/Protocols
From 2008.igem.org
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{{Imperial/Box1|Protocols| | {{Imperial/Box1|Protocols| | ||
====== 1. Transformation Protocols for integration vectors in ''Bacillus subtilis'' ====== | ====== 1. Transformation Protocols for integration vectors in ''Bacillus subtilis'' ====== | ||
| - | *[[Imperial College/Transformation |Protocol 1]] | + | *[[Team:Imperial College/Transformation |Protocol 1]] |
| - | *[[Imperial College/Transformation_2 |Protocol 2]] | + | *[[Team:Imperial College/Transformation_2 |Protocol 2]] |
====== 2. Transformation Protocol for linear DNA in ''Bacillus subtilis'' ====== | ====== 2. Transformation Protocol for linear DNA in ''Bacillus subtilis'' ====== | ||
| - | *[[/Transformation_3 |Protocol]] | + | *[[Team:Imperial College/Transformation_3 |Protocol]] |
====== 3. Transformation Protocol for ''E.coli'' ====== | ====== 3. Transformation Protocol for ''E.coli'' ====== | ||
| - | *[[Imperial College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]] | + | *[[Team:Imperial College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]] |
====== 4. Motility Analysis ====== | ====== 4. Motility Analysis ====== | ||
| - | *[[/Motility_Assay |Motility Assays]] | + | *[[Team:Imperial College/Motility_Assay |Motility Assays]] |
====== 5. PCR ====== | ====== 5. PCR ====== | ||
| - | *[[Imperial College/PCR |PCR]] | + | *[[Team:Imperial College/PCR |PCR]] |
| - | *[[Imperial College/SCPCR |Single Colony PCR]] | + | *[[Team:Imperial College/SCPCR |Single Colony PCR]] |
====== 6. Calibration Curves ====== | ====== 6. Calibration Curves ====== | ||
| - | *[[Imperial College/Calibration_Curves |Calibration Curves ]] | + | *[[Team:Imperial College/Calibration_Curves |Calibration Curves ]] |
| - | *[[/Growth |O.D.<sub>600</sub> vs cell number]] | + | *[[Team:Imperial College/Growth |O.D.<sub>600</sub> vs cell number]] |
| - | *[[/Fluorescence |Fluorescence vs GFPmut3b]] | + | *[[Team:Imperial College/Fluorescence |Fluorescence vs GFPmut3b]] |
====== 7. Promoter-RBS Testing Protocols ====== | ====== 7. Promoter-RBS Testing Protocols ====== | ||
| - | *[[/Constitutive |Constitutive Promoters and RBS]] | + | *[[Team:Imperial College/Constitutive |Constitutive Promoters and RBS]] |
| - | *[[/Inducible |pHyperspank Promoter and RBS]] | + | *[[Team:Imperial College/Inducible |pHyperspank Promoter and RBS]] |
| - | *[[/Light_Inducible |Light Inducible Promoters and RBS]] | + | *[[Team:Imperial College/Light_Inducible |Light Inducible Promoters and RBS]] |
====== 8. Biomaterial Protocols ====== | ====== 8. Biomaterial Protocols ====== | ||
| - | *[[/Biomaterial_Assay |Biomaterial Assay]] | + | *[[Team:Imperial College/Biomaterial_Assay |Biomaterial Assay]] |
|}} | |}} | ||
<br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}} | <br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}} | ||
Revision as of 14:41, 26 October 2008
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Experiments & Protocols
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