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Team:Montreal/Seeding
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| + | ==Protocol== | ||
| + | |||
| + | Seeding is a method for amplifying a single colony of genetically identical bacteria. | ||
| + | |||
| + | 1. Pipet 5.0 mL of LB broth into two 10 mL culture tube, under sterile conditions. | ||
| + | |||
| + | 2. Add the applicable antibiotic (amp+, 1 µL/mL LB; kan+, 5 µL/mL) | ||
| + | |||
| + | 3. Identify an area on the transformation plate with single colonies. "Pick" one colony by picking it up with a sterile P200 pipet tip, and eject the tip into the media of the culture tube. | ||
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| + | 4. Leave one tube with only LB and antibiotic as a control | ||
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| + | 5. Fix the caps on the tubes loosely, to allow air circulation and subsequent bacterial growth. | ||
| + | |||
| + | 6. Place the tubes in a shaking incubator at 37<sup>o</sup>C for 16-20 hours. | ||
Latest revision as of 19:40, 17 June 2008
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Protocol
Seeding is a method for amplifying a single colony of genetically identical bacteria.
1. Pipet 5.0 mL of LB broth into two 10 mL culture tube, under sterile conditions.
2. Add the applicable antibiotic (amp+, 1 µL/mL LB; kan+, 5 µL/mL)
3. Identify an area on the transformation plate with single colonies. "Pick" one colony by picking it up with a sterile P200 pipet tip, and eject the tip into the media of the culture tube.
4. Leave one tube with only LB and antibiotic as a control
5. Fix the caps on the tubes loosely, to allow air circulation and subsequent bacterial growth.
6. Place the tubes in a shaking incubator at 37oC for 16-20 hours.
