Team:Warsaw/Calendar-Main/17 June 2008

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Latest revision as of 14:47, 26 October 2008


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PCR on OmpA_linker and linker_alpha with OmpaL_N and AlphaP_XB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
PCR on OmpA_linker and linker_omega with OmpaL_N and OmegaP_EPB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
Gel electrophoresis of PCR products and gel-out of OmpA_alpha (1000 bp) and OmpA_omega (800 bp).
Electrophoresis to estimate the concentration of isolated DNA.
Overnight digest of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI (Tango 2x buffer).
Inoculation of E. coli TOP10 with pACYC177 plasmid into liquid LB + kanamycin.

Repetition of experiment from 11/06/2008.

@@ Line 5: / Line 5: @@
-<h3>Preparation of constructs with OmpA protein fusions</h3>
+<h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3>
 <h4>Michał K.</h4>
 <p><ol>
@@ Line 24: / Line 24: @@
 <li> Inoculation of  <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177>pACYC177</a> plasmid into liquid LB + kanamycin. </li>
 </ol></p>
-<h3> Blue/white and rifampicin test </h3>
+<h3> Blue/white and rifampicin test #2</h3>
 <h4>Michał L., Ewa</h4>
 <p>Repetition of experiment from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008">11/06/2008.</a></p>
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