Team:Warsaw/Calendar-Main/11 June 2008

From 2008.igem.org

(Difference between revisions)
 
(25 intermediate revisions not shown)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<html>
 +
<h3> Blue/white and rifampicin test #1</h3><h4>Michał L., Ewa, Piotr</h4>
 +
<ol>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with:
 +
<ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPMT5</a>,</li>
 +
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5-AID</a>,</li>
 +
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5-AIDT7</a>, </li>
 +
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul>
 +
<li>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</li>
-
<p>Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 and pMPMT5-AiD+T7.
 
-
Plating transformants on 'TAXI'(LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</p>
 
 +
 +
<li>Inoculation of transformants to liquid LB + Tetracycline + Ampicillin 30 μg/mL +/- 0.1% Ara. </li>
 +
</ol>
 +
</html>

Latest revision as of 14:49, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Blue/white and rifampicin test #1

Michał L., Ewa, Piotr

  1. Transformation of E. coli TOP10 carrying pZC320 with:
  2. Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
  3. Inoculation of transformants to liquid LB + Tetracycline + Ampicillin 30 μg/mL +/- 0.1% Ara.