Team:Warsaw/Calendar-Main/19 June 2008

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<h3>Preparation of constructs with OmpA protein fusions</h3>
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<h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<h3> Blue/white test </h3>
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<h3> Blue/white and rifampicin test #2 </h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
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Revision as of 14:57, 26 October 2008

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Preparation of constructs: OmpA_alpha and OmpA_omega

Michał K.

Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

Blue/white and rifampicin test #2

Piotr

  1. The analysis of lacZ sequence from pZC320:
    There is no mutation in beta-galactosidase gene. Why are the colonies white?
  2. Colony PCR.
    Template: DNA isolated from white colonies
    Primers: pZCseqL and pZCseqR


Colony PCR program
TemperatureTimeNo. of cycles
94°C4:00
94°C0:3028 cycles
48°C0:45
72°C1:30
72°C10:00
4°Cinfinite

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