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Team:Warsaw/Calendar-Main/23 June 2008
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| + | <h3> Blue/white and rifampicin test #2 </h3> | ||
| + | <h4>Piotr</h4> | ||
| + | <p>Analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br> | ||
| + | There is no mutation in lacZ gene. Why are the colonies white? Reason of white phenotype unknown; this might be chromosomal mutation, transcription supression or something else). </p> | ||
| - | <h3>Preparation of constructs | + | |
| + | |||
| + | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3> | ||
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<p> | <p> | ||
| - | Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).</p> | + | Inoculation other separate transformant colonies from plates with ligations: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha </a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> (liquid LB + kanamycin).</p> |
| - | <h3>Preparation of | + | <h3>Preparation of alpha-A and omega-A fusions</h3> |
| - | <h4>Michał L. | + | <h4>Michał L.</h4> |
| - | <p>We have successfully amplified Omega-linker (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008#fig1">Fig. 1</a>), but were unable to obtain the other | + | <p>We have successfully amplified Omega-linker (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008#fig1">Fig. 1</a>), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg<sup>2+</sup> concentration elevated to 4 mM.</p> |
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/36/PCR_Alinker_Omegalinker_WAW.jpg"width=200/></a> | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/36/PCR_Alinker_Omegalinker_WAW.jpg"width=200/></a> | ||
<var><b>Fig. 1.</b>PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.</var> | <var><b>Fig. 1.</b>PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.</var> | ||
Latest revision as of 15:00, 26 October 2008
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Blue/white and rifampicin test #2PiotrAnalysis of lacZ sequence from pZC320: Preparation of constructs: OmpA_alpha and OmpA_omegaMichał K.Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha and pACYC177+OmpA_omega (liquid LB + kanamycin). Preparation of alpha-A and omega-A fusionsMichał L.We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM. 
Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.
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