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Team:Warsaw/Calendar-Main/23 June 2008
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| - | <h3> | + | <h3> Blue/white and rifampicin test #2 </h3> |
<h4>Piotr</h4> | <h4>Piotr</h4> | ||
| - | There is no mutation in | + | <p>Analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br> |
| + | There is no mutation in lacZ gene. Why are the colonies white? Reason of white phenotype unknown; this might be chromosomal mutation, transcription supression or something else). </p> | ||
| - | + | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3> | |
| - | <h3>Preparation of constructs | + | |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<p> | <p> | ||
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<h3>Preparation of alpha-A and omega-A fusions</h3> | <h3>Preparation of alpha-A and omega-A fusions</h3> | ||
| - | <h4>Michał L. | + | <h4>Michał L.</h4> |
<p>We have successfully amplified Omega-linker (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008#fig1">Fig. 1</a>), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg<sup>2+</sup> concentration elevated to 4 mM.</p> | <p>We have successfully amplified Omega-linker (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008#fig1">Fig. 1</a>), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg<sup>2+</sup> concentration elevated to 4 mM.</p> | ||
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/36/PCR_Alinker_Omegalinker_WAW.jpg"width=200/></a> | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/36/PCR_Alinker_Omegalinker_WAW.jpg"width=200/></a> | ||
Latest revision as of 15:00, 26 October 2008
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Blue/white and rifampicin test #2PiotrAnalysis of lacZ sequence from pZC320: Preparation of constructs: OmpA_alpha and OmpA_omegaMichał K.Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha and pACYC177+OmpA_omega (liquid LB + kanamycin). Preparation of alpha-A and omega-A fusionsMichał L.We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM. 
Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.
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