Team:Warsaw/Calendar-Main/23 June 2008

From 2008.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 4: Line 4:
-
<h3> Blue/white test </h3>
+
<h3> Blue/white and rifampicin test #2 </h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
-
<p>The analysis of lacZ sequence from pZC:<br>
+
<p>Analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br>
-
There is no mutation in lacZ gene. Why the colonys are white??? Reason of white phenotype unknown (thisis must be chromosomal mutation, transcription supresion or something else)
+
There is no mutation in lacZ gene. Why are the colonies white? Reason of white phenotype unknown; this might be chromosomal mutation, transcription supression or something else). </p>
-
This is wery strange... We must make new reporter system...</p>
+
-
<h3>Cloning of protein Z DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b-OmpA-alpha</a> in place of OmpA</h3>
 
-
<h4>Weronika</h4>
 
-
<a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day.
 
-
<h3>Preparation of constructs with OmpA protein fusions</h3>
+
<h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
<p>
<p>

Latest revision as of 15:00, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Blue/white and rifampicin test #2

Piotr

Analysis of lacZ sequence from pZC320:
There is no mutation in lacZ gene. Why are the colonies white? Reason of white phenotype unknown; this might be chromosomal mutation, transcription supression or something else).

Preparation of constructs: OmpA_alpha and OmpA_omega

Michał K.

Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha and pACYC177+OmpA_omega (liquid LB + kanamycin).

Preparation of alpha-A and omega-A fusions

Michał L.

We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM.

Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.