"
Team:Warsaw/Calendar-Main/23 June 2008
From 2008.igem.org
(Difference between revisions)
PPrzanowski (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
| (3 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
| - | <h3> Blue/white test </h3> | + | <h3> Blue/white and rifampicin test #2 </h3> |
<h4>Piotr</h4> | <h4>Piotr</h4> | ||
| - | <p> | + | <p>Analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br> |
| - | There is no mutation in lacZ gene. Why | + | There is no mutation in lacZ gene. Why are the colonies white? Reason of white phenotype unknown; this might be chromosomal mutation, transcription supression or something else). </p> |
| - | + | ||
| - | |||
| - | |||
| - | |||
| - | <h3>Preparation of constructs | + | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<p> | <p> | ||
Latest revision as of 15:00, 26 October 2008
 |
|
||||||||
 |
|
||||||||
Blue/white and rifampicin test #2PiotrAnalysis of lacZ sequence from pZC320: Preparation of constructs: OmpA_alpha and OmpA_omegaMichał K.Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha and pACYC177+OmpA_omega (liquid LB + kanamycin). Preparation of alpha-A and omega-A fusionsMichał L.We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM. 
Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.
|
|
