Team:Warsaw/Calendar-Main/30 June 2008

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<h3>Preparation of constructs with OmpA protein fusions</h3><h4>Paweł</h4>
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<h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3><h4>Paweł</h4>
<p> Inoculation of <i> E. coli</i> with <A href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid on LB + ampicillin.</p>
<p> Inoculation of <i> E. coli</i> with <A href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid on LB + ampicillin.</p>

Revision as of 15:04, 26 October 2008

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Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika

  1. There were blue and white colonies but no red or green in UV
  2. Inoculation of liquid LB medium + Amp with white colonies
  3. Inoculation of 10ml LB with E.coli TOP10 carrying pZC320
  4. Inoculation of E.coli TOP10 carrying pSB1A2 standard plasmids carrying parts: BBa_E0840(Gfp genetrator) and BBa_J04450 (RFP generator)

Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

Inoculation of liquid LB medium with 6 transformant colonies.

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega

Paweł

Inoculation of E. coli with pET15b plasmid on LB + ampicillin.

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