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Team:Heidelberg/Notebook/Sensing Group/Notebook/6thweek
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== Tuesdsay, 09/09/2008 == | == Tuesdsay, 09/09/2008 == | ||
* Miniprep of LuxQ colonies no. 1,2,3,5,6 | * Miniprep of LuxQ colonies no. 1,2,3,5,6 | ||
| - | [[Image:HD 080909-PCR Miniprep-LuxQ 2.png|left|thumb| | + | [[Image:HD 080909-PCR Miniprep-LuxQ 2.png|left|thumb|180px]][[Image:HD 080909-PCR Miniprep-LuxQ 1.png|left|thumb|300px]] |
== Wednesday, 09/10/2008 == | == Wednesday, 09/10/2008 == | ||
Revision as of 17:02, 26 October 2008
Contents |
Monday, 09/08/2008
- Liquid Culture of 5 colonies from LuxQ Transformation and 1 from F1 transformation
- Miniprep of Liquid Culture from previous Transformation (done by Chenchen). Incubated for two days @ RT and about 6 h @ 37 °C
- Digestion of the Miniprep products with xbaI (Buffer2 + BSA, 1 ul enzyme @ 37 °C about 40min)
Sequencing @ GATC yielded correct sequence for LuxQ no. 1
- PCR for LuxQ and LuxP with Phusion (25 µl Mastermix, 0.5 µl each Primer, 0(1) µl DMSO, 24(23) µl H20, 0.5 µl Template)
- PCR Programme: 5 min @ 98 °C || 10 s @ 98 °C | 30 s @ 55 °C | 30 s @ 72 °C || 5 min @ 72 °C | 4 °C
Tuesdsay, 09/09/2008
- Miniprep of LuxQ colonies no. 1,2,3,5,6
