Team:Warsaw/Calendar-Main/29 August 2008

From 2008.igem.org

(Difference between revisions)

Revision as of 17:26, 26 October 2008


	Attribution analyzer / Interactive list of topics

Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Michał K.

Isolation of pET15b+A-alpha plasmids.
Control digest of isolated plasmids with XbaI and BamHI (Tango buffer).
Gel electrophoresis (Fig. 1.). Choice of proper clone.

Purification of proteins: A-alpha

Piotr

Single transformations of Rosetta with plasmids pET15b+A-alpha. Plating on LB with chloramphenicol and ampicillin.

Blue-white screening and rifampicin test in GM2163

Michał L.

GM2163 competent cells carrying pZC320 were transformed with following plasmids:

Transformants were selected on CTAXI plates (LB + Chloramphenicol + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal) because GM2163 are chloramphenicol resistant.

Fig. 1. Control XbaI/BamHI digests of plasmids isolated from transformants with colony PCR product. 1. Marker 2-4. digested plasmids pET15b+A-alpha

@@ Line 2: / Line 2: @@
 <!-- do not edit above me! -->
 <html>
-<h3>Cloning of protein A DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+Omp-alpha</a> plasmid</h3><h4>Michał K.</h4>
+<h3>Cloning of protein A DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Michał K.</h4>
 <p><ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A-alpha</a> plasmids.</li>
 <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with XbaI and BamHI (Tango buffer). </li>