Team:Heidelberg/Notebook/Sensing Group/Notebook/7thweek

From 2008.igem.org

(Difference between revisions)
(→Wednesday, 08/17/2008)
(→Thursday, 08/18/2008)
Line 136: Line 136:
== Thursday, 08/18/2008 ==
== Thursday, 08/18/2008 ==
 +
* Miniprep of LuxP
 +
* Cloning of LuxP into Fusion constructs with SalI/NotI
 +
* Transformation into DH5a competent cells
== Friday, 08/19/2008 ==
== Friday, 08/19/2008 ==

Revision as of 17:32, 26 October 2008

Back to the overview


Contents

Tuesday, 08/16/2008

  • Digestion of Fusion 1+2 with PstI
Digestion of Fusion constructs with PstI. All, except Fusion 1 colony 2 of NcoI/NdeI cloning, seem to be positive
  • F1+2 2,3 (KpnI), F1+2 1,3 (NdeI) sent to sequencing @ GATC 
F2 NdeI #3, F1 NdeI #1, F1 KpnI #2 seem to be correct

Wednesday, 08/17/2008

  • Digestion of LuxP (Glycerol-Stock and cultures from Bench) with NcoI to check if correct
Digestion of LuxP from glycerol stock and from bench culture. Both are positive.
  • O/N cultures of LuxP cells from Glycerol stock

Thursday, 08/18/2008

  • Miniprep of LuxP
  • Cloning of LuxP into Fusion constructs with SalI/NotI
  • Transformation into DH5a competent cells

Friday, 08/19/2008

Go to 8th week

Retrieved from "http://2008.igem.org/Team:Heidelberg/Notebook/Sensing_Group/Notebook/7thweek"