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Team:Heidelberg/Notebook/Sensing Group/Notebook/7thweek
From 2008.igem.org
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| - | == Tuesday, | + | == Tuesday, 09/16/2008 == |
* Digestion of Fusion 1+2 with PstI | * Digestion of Fusion 1+2 with PstI | ||
[[Image:HD 080916-F1 2 Digestion PstI.png|center|thumb|400px|Digestion of Fusion constructs with PstI. All, except Fusion 1 colony 2 of NcoI/NdeI cloning, seem to be positive]] | [[Image:HD 080916-F1 2 Digestion PstI.png|center|thumb|400px|Digestion of Fusion constructs with PstI. All, except Fusion 1 colony 2 of NcoI/NdeI cloning, seem to be positive]] | ||
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F2 NdeI #3, F1 NdeI #1, F1 KpnI #2 seem to be correct | F2 NdeI #3, F1 NdeI #1, F1 KpnI #2 seem to be correct | ||
| - | == Wednesday, | + | == Wednesday, 09/17/2008 == |
* Digestion of LuxP (Glycerol-Stock and cultures from Bench) with NcoI to check if correct | * Digestion of LuxP (Glycerol-Stock and cultures from Bench) with NcoI to check if correct | ||
[[Image:HD 080917-LuxP Digestion NcoI.png|center|thumb|400px|Digestion of LuxP from glycerol stock and from bench culture. Both are positive.]] | [[Image:HD 080917-LuxP Digestion NcoI.png|center|thumb|400px|Digestion of LuxP from glycerol stock and from bench culture. Both are positive.]] | ||
* O/N cultures of LuxP cells from Glycerol stock | * O/N cultures of LuxP cells from Glycerol stock | ||
| - | == Thursday, | + | == Thursday, 09/18/2008 == |
* Miniprep of LuxP | * Miniprep of LuxP | ||
* Cloning of LuxP into Fusion constructs with SalI/NotI | * Cloning of LuxP into Fusion constructs with SalI/NotI | ||
* Transformation into DH5a competent cells | * Transformation into DH5a competent cells | ||
| - | == Friday, | + | == Friday, 09/19/2008 == |
nothing to report | nothing to report | ||
[[Team:Heidelberg/Notebook/Sensing_Group/Notebook/8thweek | Go to 8th week]] | [[Team:Heidelberg/Notebook/Sensing_Group/Notebook/8thweek | Go to 8th week]] | ||
Revision as of 17:34, 26 October 2008
Contents |
Tuesday, 09/16/2008
- Digestion of Fusion 1+2 with PstI
- F1+2 2,3 (KpnI), F1+2 1,3 (NdeI) sent to sequencing @ GATC
F2 NdeI #3, F1 NdeI #1, F1 KpnI #2 seem to be correct
Wednesday, 09/17/2008
- Digestion of LuxP (Glycerol-Stock and cultures from Bench) with NcoI to check if correct
- O/N cultures of LuxP cells from Glycerol stock
Thursday, 09/18/2008
- Miniprep of LuxP
- Cloning of LuxP into Fusion constructs with SalI/NotI
- Transformation into DH5a competent cells
Friday, 09/19/2008
nothing to report
