Team:Warsaw/Calendar-Main/13 August 2008

From 2008.igem.org

(Difference between revisions)
Line 15: Line 15:
<li>Washing.</li>
<li>Washing.</li>
<li>Anti-rabbit antibody binding.</li>
<li>Anti-rabbit antibody binding.</li>
-
<li>Developing with BCIP and NBT (see fig.1.).</li>
+
<li>Developing with BCIP and NBT (see <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_August_2008#fig1">Fig. 1</a>).</li>
</ol>
</ol>
-
</p><img src="https://static.igem.org/mediawiki/2008/e/ec/Blot_OmpA_WAW.jpg" width=150/>
+
</p><a name="fig1"><img src="https://static.igem.org/mediawiki/2008/e/ec/Blot_OmpA_WAW.jpg" width=150/></a>
<var><b>Fig. 1.</b>Protein A detection on the surface of bacteria.<br>
<var><b>Fig. 1.</b>Protein A detection on the surface of bacteria.<br>
1 - <i>E. coli</i> expressing <b>OmpA_Omega_A_Alpha</b>,<br>
1 - <i>E. coli</i> expressing <b>OmpA_Omega_A_Alpha</b>,<br>

Revision as of 17:51, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Checking for presence of protein A on the cell membrane

Piotr

  1. Pouring bacteria by drops onto nitrocellulose.
  2. Drying.
  3. Blocking.
  4. Anti-A antibody binding.
  5. Washing.
  6. Anti-rabbit antibody binding.
  7. Developing with BCIP and NBT (see Fig. 1).

Fig. 1.Protein A detection on the surface of bacteria.
1 - E. coli expressing OmpA_Omega_A_Alpha,
2 - negative control - E. coli expressing OmpA_Z_Omega,
3 - E. coli expressing OmpA_A_Alpha,
4 - negative control - E. coli carrying OmpA_Omega_A_Alpha without inducer,
5 - E. coli expressing OmpA_A_Omega,
6 - negative control - E. coli Top10 without plasmid.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

Inoculation of pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega plasmids to LB with kanamycin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/13_August_2008"