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Team:Heidelberg/Notebook/Sensing Group/Notebook/8thweek
From 2008.igem.org
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== Thursday, 09/25/2008 == | == Thursday, 09/25/2008 == | ||
| + | * Miniprep of Fusion-LuxP-pDK48 constructs, LuxS, GFP Plasmid from Marcus | ||
| + | * Competent Cells (Top-10, UU1250) | ||
| + | * Picked colonies of Fusion-LuxP-pBAD33 | ||
== Friday, 09/26/2008 == | == Friday, 09/26/2008 == | ||
Revision as of 19:13, 26 October 2008
Contents |
Monday, 09/22/2008
- test digestion of F1_LuxP_NdeI (F1 cloned in pDK48 with NdeI), F2_LuxP_NdeI, F1_LuxP_KpnI (F1 cloned in pDK48 with KpnI) and F2_LuxP_KpnI (F2 cloned in pDK48 with KpnI) with NcoI
- Transformation of Fusion constructs (F1(NdeI)_LuxP_pDK48, F1(KpnI)_LuxP_pDK48 and F2(NdeI)_LuxP_pDK48) into MG1655 and HCB33 cells
- Preparation of cultures for Maxipreps of Fusion constructs
Tuesday, 09/23/2008
- Maxiprep of LuxP-Fusion constructs (F1(NdeI)_LuxP_pDK48, F1(KpnI)_LuxP_pDK48 and F2(NdeI)_LuxP_pDK48)
- Cloning of Fusion_LuxP in pBAD33 with BamHI/PstI
- digestions of Fusion constructs and pBAD33 as described in gel image descriptions
- O/N culture of Fusion-LuxP-pDK48 in HCB33 and MG1655 in TB-Kan
- Transformation of Fusion-LuxP-pBAD33 constructs into DH5a
Wednesday, 09/24/2008
- Glycerol-Stock of Fusion1-LuxP-pDK48 and Fusion2-LuxP-pDK48
- O/N culture of Fusion-LuxP-pDK48 in HCB33 and MG1655 in LB
- O/N culture of LuxS and GFP Plasmid from Marcus
- Re-Sequencing of Fusion-1 and Fusion-2
Correct sequence for both Fusion constructs confirmed
Thursday, 09/25/2008
- Miniprep of Fusion-LuxP-pDK48 constructs, LuxS, GFP Plasmid from Marcus
- Competent Cells (Top-10, UU1250)
- Picked colonies of Fusion-LuxP-pBAD33
