Team:Warsaw/Calendar-Main/28 August 2008

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<h3>Cloning of protein Z DNA to pET15b+Omp-alpha plasmid</h3><h4>Antoni</h4>
 
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<p><ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b+Z-alpha plasmids.</li>
 
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<li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with NdeI and NotI (Orange buffer). </li>
 
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<li>Gel elctrophoresis. Chioce of proper clone. </li>
 
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<h3>Cloning of protein A DNA to pET15b+Omp-alpha plasmid</h3><h4>Michał K.</h4>
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<h3>Cloning of protein A DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Michał K.</h4>
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<ol><li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformation pEt15b+A_alpha. <br>Primers used:
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<ol><li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformation <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a>. <br>Primers used:
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li>  
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li>  
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<li>Gel electrophoresis.</li>
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<li>Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/28_August_2008#fig1">Fig. 1.</a>).</li>
<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin.
<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin.
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/8a/Kolos.jpg" width=300/></a> <var><b>Fig. 1.</b> PCR on colonies from plates with transformation<br>
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1. Marker<br>
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2-23. PCR products<br></var>
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Latest revision as of 19:15, 26 October 2008

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Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Michał K.

  1. Colony PCR on colonies from plates with transformation pET15b+A_alpha.
    Primers used: AP+NotI and AL+SacI.
  2. Gel electrophoresis (Fig. 1.).
  3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.
Fig. 1. PCR on colonies from plates with transformation
1. Marker
2-23. PCR products

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