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Team:Warsaw/Calendar-Main/29 August 2008
From 2008.igem.org
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https://static.igem.org/mediawiki/2008/a/a8/Kokodak.jpg" width=300/></a> <var><b>Fig. 1.</b> Control XbaI/BamHI digests of plasmids isolated from transformants with colony PCR product.<br> | https://static.igem.org/mediawiki/2008/a/a8/Kokodak.jpg" width=300/></a> <var><b>Fig. 1.</b> Control XbaI/BamHI digests of plasmids isolated from transformants with colony PCR product.<br> | ||
1. Marker<br> | 1. Marker<br> | ||
| - | 2-4. | + | 2-4. Digested plasmids pET15b+A-alpha <br></var> |
Revision as of 19:17, 26 October 2008
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Purification of proteins: A-alphaPiotrSingle transformations of Rosetta with plasmids pET15b+A-alpha. Plating on LB with chloramphenicol and ampicillin. Blue-white screening and rifampicin test in GM2163Michał L.GM2163 competent cells carrying pZC320 were transformed with following plasmids: Transformants were selected on CTAXI plates (LB + Chloramphenicol + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal) because GM2163 are chloramphenicol resistant. Cloning of protein A DNA to pET15b+OmpA-alpha plasmidMichał K.
 Fig. 1. Control XbaI/BamHI digests of plasmids isolated from transformants with colony PCR product.1. Marker 2-4. Digested plasmids pET15b+A-alpha
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