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Team:Warsaw/Calendar-Main/14 August 2008
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<h3>Purification of proteins: Z-alpha and Z-omega</h3><h4>Piotr</h4> | <h3>Purification of proteins: Z-alpha and Z-omega</h3><h4>Piotr</h4> | ||
| - | <p>Electrophoresis in polyacrylamide gel (12 %) of sonicate and preliminarily purified protein | + | <p>Electrophoresis in polyacrylamide gel (12 %) of sonicate and preliminarily purified protein fusions: Z-alpha and Z-omega (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008#fig1">Fig. 1.</a> and <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008#fig2">Fig. 2.</a>)</p><br> |
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/35/August_14_th_2.jpg" width=250 /></a><var><b>Fig. 1.</b> Fractions from purifying Z-alpha and Z-omega protein fusions.<br> | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/35/August_14_th_2.jpg" width=250 /></a><var><b>Fig. 1.</b> Fractions from purifying Z-alpha and Z-omega protein fusions.<br> | ||
Revision as of 20:42, 26 October 2008
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Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.
Purification of proteins: Z-alpha and Z-omegaPiotrElectrophoresis in polyacrylamide gel (12 %) of sonicate and preliminarily purified protein fusions: Z-alpha and Z-omega (Fig. 1. and Fig. 2.)  Fig. 1. Fractions from purifying Z-alpha and Z-omega protein fusions.1. purified Z-omega 2. supernatant Z-alpha after sonication 3. elution of Z-alpha using ddH2o 4. purified Z-alpha 5. Marker  Fig. 2. Sonicate and pellet from Z-omega induction1. Marker 2. sonicate Z-omega after induction 3. pellet Z-omega after induction
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