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Team:Warsaw/Calendar-Main/20 August 2008
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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | <h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | ||
| - | <ol><li>Inoculation of all OmpA variants of constructs to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and | + | <ol><li>Inoculation of all pACYC177-OmpA variants of constructs to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and 100 μg/ml of protein (Z-alpha and Z-omega; negative control without protein).</li> |
<li>Measurement of growth after 5 hours and on next day.</li></ol> | <li>Measurement of growth after 5 hours and on next day.</li></ol> | ||
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<h3>Cloning of alpha to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_Z_omega</a></h3><h4>Michał K.</h4> | <h3>Cloning of alpha to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_Z_omega</a></h3><h4>Michał K.</h4> | ||
| - | <p> Colonies from both plates(<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha-A-omega>pACYC177+OmpA_alpha_A_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BOmpA-alpha-Z-omega>pACYC177+OmpA_alpha_Z_omega</a>) was inoculated to liquid LB with kanamycin (only two colonies grown on each plate).</p> | + | <p> Colonies from both plates(<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha-A-omega>pACYC177+OmpA_alpha_A_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BOmpA-alpha-Z-omega>pACYC177+OmpA_alpha_Z_omega</a>) was inoculated to liquid LB with kanamycin (only two colonies have grown on each plate).</p> |
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Tests for ampicillin resistance given by protein added to mediumPiotr
Cloning of protein A DNA to GeneArt plasmidAntoniTransformation of E. coli TOP10 strain with ligation products from previous day. Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.Colonies from both plates(pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) was inoculated to liquid LB with kanamycin (only two colonies have grown on each plate).
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