Team:Warsaw/Calendar-Main/25 August 2008

From 2008.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 6: Line 6:
<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
-
<p> <ol><li>Purification OmpA_alpha of antibiotic resistance contamination:<br>
+
<p> <ol><li>Purification of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> from antibiotic resistance contamination:<br>
-
Inoculation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  careing pACTC177+OmpA_alpha to liquin LB with kanamycin and with kanamycin and ampicylin.</li>
+
Inoculation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  carrying pACTC177+OmpA_alpha to liquid LB with kanamycin and with kanamycin and ampicillin.</li>
-
<li>Inoculation of TOP10 carrying OmpA_A_alpha and OmpA-alpha to LB with inductor (0.25 mM IPTG) and kanamycin.</p>
+
<li>Inoculation of TOP10 carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA-alpha</a> to LB with inductor (0.25 mM IPTG) and kanamycin.</p>
</li></ol>
</li></ol>
Line 14: Line 14:
-
<h3>Cloning of protein A DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> plasmid</h3><h4>Antoni</h4>
+
<h3>Cloning of protein A DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Antoni</h4>
<p><ol>
<p><ol>
Line 26: Line 26:
<h3>Cloning of alpha to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a></h3><h4>Michał K.</h4>
<h3>Cloning of alpha to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a></h3><h4>Michał K.</h4>
-
<p> Eight colonies from each plate (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha-A-omega>pACYC177+OmpA_alpha_A_omega</a> and <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BOmpA-alpha-Z-omega>pACYC177+OmpA_alpha_Z_omega</a>) was inoculated to liquid LB with kanamycin.</p>
+
<p> Eight colonies from each plate (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha-A-omega>pACYC177+OmpA_alpha_A_omega</a> and <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BOmpA-alpha-Z-omega>pACYC177+OmpA_alpha_Z_omega</a>) were inoculated to liquid LB with kanamycin.</p>
</html>
</html>

Latest revision as of 20:53, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Tests for ampicillin resistance given by protein added to medium

Piotr

  1. Purification of pACYC177+OmpA_alpha from antibiotic resistance contamination:
    Inoculation of E. coli TOP10 carrying pACTC177+OmpA_alpha to liquid LB with kanamycin and with kanamycin and ampicillin.
  2. Inoculation of TOP10 carrying pACYC177+OmpA_A_alpha and pACYC177+OmpA-alpha to LB with inductor (0.25 mM IPTG) and kanamycin.

Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Antoni

  1. Transformation of E. coli TOP10 strain with friday's ligation products.
  2. Transformants plating on LB + ampicillin.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

Eight colonies from each plate (pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) were inoculated to liquid LB with kanamycin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/25_August_2008"