Team:Warsaw/Calendar-Main/27 August 2008

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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
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Results of growth from previus day
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Results of growth from previous day
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https://static.igem.org/mediawiki/2008/1/1b/Go29.jpg" width=300/></a> <var><b>Fig. 1.</b> NdeI/NotI digest of plasmid pGeneart+A<br>  
https://static.igem.org/mediawiki/2008/1/1b/Go29.jpg" width=300/></a> <var><b>Fig. 1.</b> NdeI/NotI digest of plasmid pGeneart+A<br>  
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2. digested plasmid pGeneart+A<br></var>
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2. Digested plasmid pGeneart+A<br></var>
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Latest revision as of 20:56, 26 October 2008

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Tests for ampicillin resistance given by protein added to medium

Piotr

Results of growth from previous day

OmpA variant ("hunter")"Prey" variantGrowth with preyGrowth without prey
OmpA-alphaHis+Z+omega+-
OmpA-alphaHis+Z+alfa--
OmpA-A-alphaHis+Z+omega+-

Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Michał K.

  1. Digest of pGeneart+A and pET15b+OmpA-alpha with NdeI and NotI (Orange buffer), pET15b was also dephosphorylated.
  2. Gel electrophoresis of digested plasmids (Fig. 1.) and gel-out of proper bands (400 bp for A lane and 6200 bp for pET15b lane).
  3. Ligation of pET15b-Alpha and A.
  4. Transformation of E. coli TOP10.
  5. Transformants plating on LB + ampicillin.
    6. Fig. 1. NdeI/NotI digest of plasmid pGeneart+A
    1. Marker
    2. Digested plasmid pGeneart+A




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