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Team:Warsaw/Calendar-Main/29 August 2008
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<h4>Piotr</h4> | <h4>Piotr</h4> | ||
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| - | + | Transformation of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A-alpha</a> plasmid. Plating on LB with chloramphenicol and ampicillin.</p> | |
<h3>Blue-white screening and rifampicin test in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#gm">GM2163</a></h3> | <h3>Blue-white screening and rifampicin test in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#gm">GM2163</a></h3> | ||
Latest revision as of 20:58, 26 October 2008
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Purification of proteins: A-alphaPiotrTransformation of Rosetta with pET15b+A-alpha plasmid. Plating on LB with chloramphenicol and ampicillin. Blue-white screening and rifampicin test in GM2163Michał L.GM2163 competent cells carrying pZC320 were transformed with following plasmids: Transformants were selected on CTAXI plates (LB + Chloramphenicol + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal) because GM2163 are chloramphenicol resistant. Cloning of protein A DNA to pET15b+OmpA-alpha plasmidMichał K.
 Fig. 1. Control XbaI/BamHI digests of plasmids isolated from transformants with colony PCR product.1. Marker 2-4. Digested plasmids pET15b+A-alpha
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